LC-MS/MS法测定血浆中利奈唑胺浓度  

Determination the concentration of linezolid in human plasma by HPLC-MS/MS

作　　者：孙纯广[1] 白楠[2] 蔡芸[2] 梁蓓蓓[2] 张述雷[2] 王睿[2] 史录文[1] 

机构地区：[1]北京大学医学部药事管理与临床药学系,北京100191 [2]解放军总医院临床药理研究室,北京100853

出　　处：《中国临床药理学杂志》2010年第10期772-776,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的建立高效液相色谱-串联质谱法测定人血浆中利奈唑胺(新型唑烷酮类抗菌药物)浓度的方法。方法以呋喃唑酮作为内标,血浆样品经乙腈沉淀蛋白提取分离。结果利奈唑胺测定在20～4000ng·mL-1线性良好,最低定量限为20ng·mL-1,提取回收率在90%～98%,日内、日间精密度均在88.53%～110.7%,准确度(RSD)≤6.83%。结论建立的LC-MS/MS方法专属性强,灵敏度高,操作较以往简洁、快速、定量准确、实用性强,适合于利奈唑胺药代动力学测定。Objective To establish a HPLC - MS/MS method for determining linezolid concentration in human plasma. Methods With fura- zolidone as internal standard, plasma samples extracted by acetonitrile. Results Determination of linezolid had good linearity in the 20 -4000 ng·mL-1 , lower limit of quantification was 20 ng mL-1, extraction recovery was 90% to 98% , accuracy was 88.53% to 110. 7% , and precision （ RSD ）≤6.83%. Conclusion The established LC - MS/MS method is specific, sensitive, and simple, fast, accurate and suitable for determination of Linezolid in human plasma.

关 键 词：利奈唑胺 液相色谱质谱法 血浆 

分 类 号：R978.1[医药卫生—药品] R969.1[医药卫生—药学]