利用生物条形码技术对蓖麻毒素进行微量检测  

Bio-bar codes assay(BCA) for the ultrasensitive detection of ricin

作　　者：冯玉奎[1] 张立营[1] 孙宇 孙英姿[1] 

机构地区：[1]济南军区青岛第二疗养院检验科,山东青岛266071 [2]305医院检验科,北京100050

出　　处：《实用医药杂志》2010年第10期928-931,共4页Practical Journal of Medicine & Pharmacy

基　　金：青岛市公共领域科技支撑计划项目(09-1-1-28-nsh)

摘　　要：目的建立蓖麻毒素的高灵敏检测方法。方法利用蓖麻毒素的多抗及特异DNA链标记的金纳米颗粒探针(NP)和蓖麻毒素单抗标记的磁性微球探针(MMP),形成MMP-蓖麻毒素-NP三明治复合物后再利用去杂交将NP探针上标记的DNA链释放出来,通过PCR方法或芯片检测方法鉴定这些释放的DNA链确定蓖麻毒素的存在。结果建立了蓖麻毒素的生物条形码检测体系,检测灵敏度可达1fg/ml。和临床上常规的检测方法相比,其检测灵敏度可达常规ELISA的106倍。结论生物条形码技术可作为蓖麻毒素一种高灵敏度的检测方法。Objective To establish a highly sensitive method for detecting ricin.Method NPs（nanoparticle probes） that are encoded with DNA that is unique to ricin and polyclonal antibodies against ricin and MMPs（magnetic microparticle probes） with monoclonal antibodies that specifically bind ricin were prepared.After forming MMP-ricin-NP sandwich complex,dehybridization of the oligonucleotides on the nanoparticle probe surface allows the determination of the presence of ricin by identifying the oligonucleotide sequence released from the nanoparticle probe through PCR or chip-based detection.Result The Bio-bar codes assay system for detecting ricin was established,and the sensitivity limit was about 1 fg/ml;Compared with clinically accepted conventional ELISA assays for detecting the same target,six orders of magnitude of above-mentioned method more sensitive than what was observed with ELISA.Conclusion This study lays a foundation for establishing highly sensitive Ricin Bio-bar Codes Assay kit.

关 键 词：蓖麻毒素 生物条形码检测 

分 类 号：R977.6[医药卫生—药品] R979.5[医药卫生—药学]