绿花重瓣铁线莲愈伤组织诱导研究  

Inducement of Clematis florida var.plena Callus

作　　者：张丽琼[1] 常权记[1] 廖咸康[1] 周琼[1] 禚苏[1] 

机构地区：[1]安康学院农学与生命科学院,陕西安康725000

出　　处：《北方园艺》2010年第20期152-154,共3页Northern Horticulture

基　　金：安康学院专项科研计划资助项目(2005AZX019)

摘　　要：以绿花重瓣铁线莲茎段为外植体,MS为基本培养基,研究了2,4-D与6-BA对绿花重瓣铁线莲愈伤组织诱导效应的影响。结果表明:2种生长调节剂单独使用时,均能诱导出愈伤组织,且2种愈伤组织诱导的部位及形态有明显的不同,2,4-D诱导的部位为茎段中部,愈伤组织白色膨松,6-BA诱导的部位为茎段顶端,愈伤组织浅绿色疏松;2,4-D与6-BA配合使用时,相互存在拮抗作用,不利于愈伤组织的诱导。The effect of 2,4-D and 6-BA to the callus inducement of Clematis florida var.plena were studied in this experiment,it had used the young stem to be the explants and used the MS medium to be the base medium.The results showed that the two plant growth regulators both can inducement callus when use them alone,and the growing position and form of the two callus was different distinctly.The callus of inducement by 2,4-D was white and bulginess,and its growing position was the middle of the stem.The callus of inducement by 6-BA was light green and loose,and its growing position was the top of the stem.It was disadvantageous to the callus inducement when the two plant growth regulators being used altogether,maybe the two plant growth regulators can resist each other.

关 键 词：绿花重瓣铁线莲 愈伤组织 诱导 

分 类 号：S687.3[农业科学—观赏园艺]