三种药用植物内生菌的分离及其抗肿瘤活性菌株的筛选  

Isolation and Screening for Endophyte with Antitumor Activities from Three Medicinal Plants

作　　者：陈传文[1,2] 孙前光[1] 朱军[1] 吴晓鹏[1] 黄惠琴[1] 鲍时翔[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571101 [2]海南大学农学院,海南海口570228

出　　处：《微生物学通报》2010年第10期1462-1466,共5页Microbiology China

基　　金：中央级公益性科研院所基金项目(No.ITBBZD2007-5-1)

摘　　要：对204株分离自长春花、海南粗榧和见血封喉的内生菌进行了抗肿瘤活性筛选。结果显示,有19株内生菌的发酵液至少对一种指示瘤株具有细胞毒活性,其中4株菌的发酵液对S180的抑制率较强,均在70%以上。研究表明菌株PA09006和PA09009发酵液中的抗肿瘤活性物质,具有一定的酸碱耐受性,并且对温度和紫外线具有一定的稳定性。Two hundred and four endophytes isolated from Catharanthus roseus,Cephalotaxus hainanensis and Antiaris toxicaria were screened for antitumor activities.The results showed that broth of 19 strains presented cytotoxic activities to at least one of the tested tumor cells,four of them exhibited strong antitumor activity against S180,were above 70%.Studies have shown that broth in the antitumor active substances of strains PA09006 and PA09009,confer a certain to pH value and ultraviolet.

关 键 词：长春花 海南粗榧 见血封喉 内生菌 细胞毒活性 

分 类 号：R284[医药卫生—中药学]