白花泡桐内生真菌的分离鉴定及抗菌活性筛选  

Isolation and Antimicrobial Activity of Endophytic Fungi from Paulownia fortunei

作　　者：罗江华[1] 吾鲁木汗.那孜尔别克 李科[1] 姚福成 恩特马克.布拉提白 

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000 [2]湘西宏成制药有限责任公司,湖南吉首416000

出　　处：《微生物学通报》2010年第10期1481-1485,共5页Microbiology China

基　　金：吉首大学研究生科研创新项目(No.YGY200910);湘西宏成制药有限责任公司科技开发项目(No.XHZYP200921)

摘　　要：从白花泡桐根、茎、叶中分离出19株内生真菌,对分离出的真菌分别利用琼脂块法和纸片扩散法进行初选和复选,通过形态学特征观察和rRNA基因ITS序列系统发育分析对抗菌活性菌株进行鉴定。抑菌试验结果显示JSD-8、JM-1和JM-10等3株内生真菌对枯草芽胞杆菌(Bacillus subtilis)CGMCC1.769、大肠杆菌(Escherichia coli)CGMCC1.1103和白色念珠菌(Candida albi-cans)ATCC10123均有一定的抗菌活性。鉴定结果表明,JSD-8、JM-1、JM-10分别属于串珠赤霉菌(Gibberella moniliformis)、长柄链格孢菌(Alternaria longipes)和托姆青霉菌(Penicilium thomii)。Nineteen strains of endophytic fungi were isolated from the stems,roots and leaves of Paulownia fortune,their antimicrobial activity against three tested microorganisms were investigated by using the agar diffusion method and the paper disc method.Identification of these strains were determined by their morphological features and phylogenetic analysis based on internal transcribed spacer of rRNA gene sequence comparison.The results showed that endophytic fungi of JSD-8,JM-1 and JM-10 had remarkable antimicrobial activities to Bacillus subtilis CGMCC 1.769,Escherichia coli CGMCC 1.1103 and Candida albicans ATCC 10123,respectively.Fungal morphological and molecular identification demonstrated that JSD-8,JM-1 and JM-10 represented Gibberella moniliformis,Alternaria longipes and Penicilium thomii,respectively.

关 键 词：白花泡桐 内生真菌 抗菌活性 鉴定 

分 类 号：Q93[生物学—微生物学]