Identification and expression analysis of mical family genes in zebrafish  

作　　者：Yulin Xue Chikin Kuok An Xiao Zuoyan Zhu Shuo Lin Bo Zhang 

机构地区：[1]Key Laboratory of Cell Proliferation and Differentiation, Center of Developmental Biology and Genetics, College of Life Sciences, Peking University, Ministry of Education, Beijing 100871, China [2]Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095-1606, USA

出　　处：《Journal of Genetics and Genomics》2010年第10期685-693,共9页遗传学报（英文版）

基　　金：supported by National Natural Science Foundation of China (Nos. 30721064 and 30730056, 30620120101);National Basic Research Program of China (973 Program) (Nos. 2005CB522504, 2006CB943801 and 2007CB914502)

摘　　要：Mical （molecule interacting with CasL） represent a conserved family of cytosolic multidomain proteins that has been shown to be as- sociated with a variety of cellular processes, including axon guidance, cell movement, cell-cell junction formation, vesicle trafficking and cancer cell metastasis. However, the expression and function of these genes during embryonic development have not been comprehen- sively characterized, especially in vertebrate species, although some limited in vivo studies have been carried out in neural and muscula- ture systems of Drosophila and in neural systems of vertebrates. So far, no mical family homologs have been reported in zebrafish, an ideal vertebrate model for the study of developmental processes. Here we report eight homologs of mical family genes in zebrafish and their expression profiles during embryonic development. Consistent with the findings in Drosophila and mammals, most zebra_fish mical family genes display expression in neural and musculature systems. In addition, five mical homologs are detected in heart, and one, mi- call2a, in blood vessels. Our data established an important basis for further functional studies of mical family genes in zebrafish, and suggest a possible role for mical genes in cardiovascular development.Mical （molecule interacting with CasL） represent a conserved family of cytosolic multidomain proteins that has been shown to be as- sociated with a variety of cellular processes, including axon guidance, cell movement, cell-cell junction formation, vesicle trafficking and cancer cell metastasis. However, the expression and function of these genes during embryonic development have not been comprehen- sively characterized, especially in vertebrate species, although some limited in vivo studies have been carried out in neural and muscula- ture systems of Drosophila and in neural systems of vertebrates. So far, no mical family homologs have been reported in zebrafish, an ideal vertebrate model for the study of developmental processes. Here we report eight homologs of mical family genes in zebrafish and their expression profiles during embryonic development. Consistent with the findings in Drosophila and mammals, most zebra_fish mical family genes display expression in neural and musculature systems. In addition, five mical homologs are detected in heart, and one, mi- call2a, in blood vessels. Our data established an important basis for further functional studies of mical family genes in zebrafish, and suggest a possible role for mical genes in cardiovascular development.

关 键 词：mical micall2a expression pattern blood vessel HEART 

分 类 号：Q42[生物学—神经生物学] S511[生物学—生理学]