Expression of CENH3 alleles in synthesized allopolyploid Oryza species  

作　　者：Hui Li Li Lu Yanfang Heng Rui Qin Yongzhong Xing Weiwei Jin 

机构地区：[1]National Maize Improvement Center of China, Key Laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture,Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China [2]National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China [3]Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Sciences, South-Central University for Nationalities, Wahan 430074, China

出　　处：《Journal of Genetics and Genomics》2010年第10期703-711,共9页遗传学报（英文版）

基　　金：supported by the Natural Science Foundation of China (No. 31025018);by the Program for National Transgenic Research Program of China (No. 2009ZX08010-00313) to W.W. Jin.

摘　　要：Synthesized allopolyploids are valuable materials for comparative analyses of two or more distinct genomes, such as the expression changes （activation, inactivation or differential expression） of orthologous genes following allopolyploidization. CENH3 is a centromerespecific histone H3 variant and has been regarded as a central component in kinetochore formation and centromere function. In this study, interspecific hybrids of Oryza genus （AA × CC, AA × CCDD） and their backcross progenies were produced, and the genome constitutions were identified as AC, ACC, ACD, AACD, or AA（CD） by Genomic in situ hybridization （GISH）. We further cloned and sequenced the CENH3 genes from O. sativa （AA）, O. officinalis （CC） and O. latifolia （CCDD）. Sequencing of RT-PCR products revealed that CENH3_C2 and CENH3_D, the two CENH3 alleles from O. latifolia, showed polymophism in several sites, while CENH3_C2 and CENH3_C1 from O. officinalis were different at only two amino acids positions. Moreover, we found that the CENH3 genes from both parents are expressed in interspecific hybrids and their progenies. Specifically, based on our cDNA sequencing data, the ratio of expres- sion level between CENH3_A and CENH3_C1 was approximately 1 in AC and 0.5 in ACC genomes, respectively. As a result, the CENH3 expression patterns shed more light on the inter-coordination between varied centromeric DNA sequences and highly conserved kinetochore protein in synthesized allopolyploids of Oryza genus.Synthesized allopolyploids are valuable materials for comparative analyses of two or more distinct genomes, such as the expression changes （activation, inactivation or differential expression） of orthologous genes following allopolyploidization. CENH3 is a centromerespecific histone H3 variant and has been regarded as a central component in kinetochore formation and centromere function. In this study, interspecific hybrids of Oryza genus （AA × CC, AA × CCDD） and their backcross progenies were produced, and the genome constitutions were identified as AC, ACC, ACD, AACD, or AA（CD） by Genomic in situ hybridization （GISH）. We further cloned and sequenced the CENH3 genes from O. sativa （AA）, O. officinalis （CC） and O. latifolia （CCDD）. Sequencing of RT-PCR products revealed that CENH3_C2 and CENH3_D, the two CENH3 alleles from O. latifolia, showed polymophism in several sites, while CENH3_C2 and CENH3_C1 from O. officinalis were different at only two amino acids positions. Moreover, we found that the CENH3 genes from both parents are expressed in interspecific hybrids and their progenies. Specifically, based on our cDNA sequencing data, the ratio of expres- sion level between CENH3_A and CENH3_C1 was approximately 1 in AC and 0.5 in ACC genomes, respectively. As a result, the CENH3 expression patterns shed more light on the inter-coordination between varied centromeric DNA sequences and highly conserved kinetochore protein in synthesized allopolyploids of Oryza genus.

关 键 词：synthesized allopolyploid Oryza genus CENH3 gene expression 

分 类 号：Q943[生物学—植物学] Q78