杜仲组织培养中培养基与含胶量关系的研究  

A Study on Relation between Medium and Gutta Cotent inTissue Culture of Eucommia ulmoides Oliv.

作　　者：杨振堂[1] 臧埔 胡桂珍[1] 马淑琴[1] 

机构地区：[1]中国农业科学院特产研究所

出　　处：《特产研究》1999年第1期1-5,共5页Special Wild Economic Animal and Plant Research

基　　金：吉林省科委资助

摘　　要：以杜仲（ＥｕｃｏｍｍｉａｕｌｍｏｉｄｅｓＯｌｉｖ．）幼苗的茎、叶为外植体，在附加植物激素的ＭＳ、Ｂ５和Ｈ培养基上，诱导出了大量的愈伤组织（平均诱导率９４．２％）。在继代培养中建立了３个愈伤组织无性系。绝大多数愈伤组织系以橡胶烃表示的杜仲胶含量都超过了对照（幼苗茎、叶）的５０％。Ｂ５培养基适宜于生产杜仲胶，附加激素Ｂ有利于提高含胶量。愈伤组织系的平均含胶量５．６２％，为对照的５９．４％。The stem and leaf of the seedlings of Eucommia ulmoides Oliv.being used as explants ,a large amount of callus was induced on MS,B5 and H medium supplemented with plant hormones with an average induction frequency of 94.2% .Three callus clones were established in the course of the subculture of callus.Gutta content that was expressed as rubber-hydrocarbon content exceeded the control's (the stem and leaf of seedlings)by 50% for the overwhelming majority of callus clones.B5 medium was suitable for the production of gutta and hormone B was advantageous to the increase of gutta content.An average gutta content of the callus clones was 5.62% or 59.4% of the control's.

关 键 词：杜仲 组织培养 杜仲胶 

分 类 号：S567.15[农业科学—中草药栽培]