利用混合抗血清检测苹果潜隐病毒  

DETECTION OF APPLE CHLOROTIC LEAFSPOT VIRUS AND STEM GROOVING VIRUS WITH AN ANTISERUM MIXTURE

作　　者：李春敏[1] 章德明[1] 

机构地区：[1]河北省农林科学院昌黎果树研究所

出　　处：《河北林果研究》1999年第1期78-80,共3页Hebei Journal of Forestry and Orchard Research

摘　　要：为寻求判断苗木是否带毒的简便方法，利用ＰＡＳ—ＥＬＩＳＡ检测方法，将ＡＣＬＳＶ和ＡＳＧＶ的抗血清混合，并同时加入同一检测孔中，定性检测苹果组培苗ＡＣＬＳＶ和ＡＳＧＶ的带毒情况。发现该方法在检测这两种病毒的混合毒源时，比采用单一抗血清检测灵敏度高，且费用较低。Rabbit antisera against apple chlorotic leafspot virus(CLSV) and apple stem grooving virus(ASGV) were mixed up.And the mixture was employed to detect the in vitro shoot samples by protein A sandwich enzyme linked immunobent assay(PAS ELISA) Comparative assays were performed by PAS ELISA with each of the antisera The results indicated that the antiserum mixture was more sensitive in detecting the viruses in the samples infected by both CLSV and ASGV

关 键 词：苹果 病毒 检测 抗血清 

分 类 号：S436.611.1[农业科学—农业昆虫与害虫防治]