基于复合智能控制的非接触式液体定量分配系统  

Non-contact Liquid Rationing System Based on Compound Intelligent Control

作　　者：刘亚欣[1,2] 陈立国[2] 孙立宁[2] 

机构地区：[1]哈尔滨工业大学仪器科学与技术博士后流动站,哈尔滨150001 [2]哈尔滨工业大学机器人技术与系统国家重点实验室,哈尔滨150001

出　　处：《机械工程学报》2010年第20期175-181,共7页Journal of Mechanical Engineering

基　　金：国家高技术研究发展计划(863计划;2007AA04Z311;2007AA04Z336);卫生部重大专项(2009ZX10004-719);威海市基金(2009-3-93);哈尔滨工业大学(威海)校科学研究基金(HIT(WH)-XBQD201006)资助项目

摘　　要：为解决生物、化学、医药等领域通量化试验操作中不同种类、体积液体试剂频繁操作过程中精度、灵活性降低等问题,满足微量、精确、快速灵活的试剂定量分配要求,研制集成MEMS微流量传感器的具有分配液体体积自适应调整功能的通量化液体非接触式分配系统。介绍系统的组成原理,提出基于复合智能模糊控制的分配体积闭环控制方法,减小系统延迟、流速波动和剩余试剂体积动态变化等因素对分配精度的影响,实现通量化试剂分配过程中控制参数的自校准和分配体积的精确控制;针对具有不同粘度、期望体积的液体试剂进行分配试验,验证复合智能模糊控制方法的优越性。结果表明采用该控制方法后该系统分配1μL以上体积试剂时重复精度(用变异系数表示)小于3%;当分配体积100nL时,分配重复精度小于4%,具有较好的分配重复精度。In biomedical areas,thousands of reagents with different viscosities and various desired volume need to be dispensed in submicroliter range simultaneously.Therefore,most of the commercial automated liquid dispensing systems need to be calibrated through experiment to accurately dispense liquids when the reagents viscosities change,which is time consuming and not accurate enough.A high-throughput self-adjusted precise liquid handling system is developed,in which a MEMS flow sensor is used.The system construction is introduced in detail.A novel closed-loop control strategy based on compound intelligent fuzzy control method is proposed to calculate the valve open time in each dispensing cycle,which makes the system immune to liquid viscosity,pressure fluctuation,and other sources of error.Dispensing results show that the system can achieve better dispensing performance,and the coefficient of variance（CV） for liquid dispensing is below 3% at 1 μL and below 4% at 100 nL.

关 键 词：微量试剂分配 智能模糊控制 MEMS流量传感器 

分 类 号：TG242[金属学及工艺—铸造]