痛风性关节炎患者外周血单个核细胞NLRP3炎性体mRNA表达的研究  

Expression of NLRP3 inflammasome mRNA in peripheral blood monocytes of patients with gouty arthritis

作　　者：杨其彬[1] 周京国[1] 青玉凤[1] 谢文光[1] 赵明才[1] 李敏[1] 

机构地区：[1]川北医学院附属医院风湿免疫研究所,南充637000

出　　处：《中华风湿病学杂志》2010年第10期686-689,共4页Chinese Journal of Rheumatology

基　　金：基金项目：四川省科技厅科研基金（2009JY0166）;四川省卫生厅科研基金（070289）

摘　　要：目的 检测痛风性关节炎（GA）患者外周血单个核细胞（PBMCs）中NLRP3炎性体[NLRP3、凋亡相关斑点样蛋白（ASC）、半胱天冬酶（cagpase）-1] mRNA表达水平,探讨其在GA发病机制中可能的作用方法用实时荧光定量聚合酶链反应（PCR）法检测24例GA患者和24名健康体检者PBMCs中NLRP3炎性体mRNA的表达水平,并与疾病相关实验室指标进行分析.组间比较采用t检验,相关性分析采用Spearman相关分析.结果 ASC mRNA在GA组的表达量显著高于健康对照组[分别为（0.029±0.021）和（0.009±0.007）,P＜0.01],而GA组NLRP3、caspase-1 mRNA的表达量均明显低于健康对照组[分别为（0.062±0.084）和（0.133±0.106）.P＜0.05;（0.025±0.014）和（0.117±0.156）,P＜0.01].GA患者ASCmRNA的表达量与球蛋白、极低密度脂蛋白胆醇呈负相关（r分别为-0.547、-0.540,P均＜0.05）,caspase-1mRNA的表达量与球蛋白、极低密度脂蛋白胆固醇呈负相关（r分别为-0.773、-0.465,P＜0.01或P＜0.05）.GA患者ASC mRNA表达量与NLRP3 mRNA呈负相关（r=-0.450,P＜0.05）,与caspase-1 mRNA呈正相关（r=0.604,P＜0.01）.结论 NLRP3炎性体在GA患者中表达异常,提示 NLRP3炎性体可能参与了痛风炎症反应过程,在痛风炎症机制中发挥重要作用.Objective To study the expression level of NLRP3 （NLR family, pyrin domain containing3） inflammasome （NLRP3, ASC, caspase-1 ） mRNA in peripheral blood monocytes （PBMCs） from patients with gout arthritis （GA） and to explore the pathogenesis of GA. Methods NLRP3 inflammasome mRNA was measured using quantitative real-time PCR in PBMCs. The expression of NLRP3 inflammasome mRNA in PBMCs was compared between patients with GA （n=24） and healthy controls （n=24）. β-actin was selected as the internal control. Students＇ t-test was used in two independent samples and Spearman＇s correlation was used to evaluate the relationship between mRNA level and inflammatory parameters. Results The expression of ASC mRNA in GA increased significantly when compared to healthy controls [（0.029±0.021 ） vs （0.009±0.007 ）, P〈0.01 ], and the expression of NLRP3 and caspase-1 mRNA was significantly lower in patients with GA compared to healthy controls [（0.062±0.084） vs （0.133±0.106）, P〈0.05; （0.025±0.014） vs （0.117±0.156）, P〈0.01]. Moreover, the expression of ASC mRNA was found to correlate significantly with globulin （r=-0.547, P〈0.05） and very low density lipoprotein cholesterol （r=-0.540, P〈0.05） in GA patients,and caspase-1 was correlated to globulin（r= -0.773. P〈0.01） and very low density lipoprotein cholesterol （r=-0.465. P〈0.05）. Furthermore, the ASC mRNA in GA patients was associated significantly with NLRP3 mRNA（r=-0.450, P〈0.05） and caspase-1 （r=0.604, P〈0.01 ）. Conclusion Dysregulated expression of the NLRP3inflammasome is involved in the inflammatory response and plays a key role in the pathogenesis of GA.

关 键 词：关节炎 痛风性 赞陛体 NLRP3 凋亡相关斑点样蛋白 半胱天冬酶-1 

分 类 号：R686[医药卫生—骨科学]