试验设计优化超声提取千斤拔酚酸（英文）  

Optimization of Ultrasonic Extraction of Phenolics from Radix Flemingia philippinensis by Statistical Design of Experiment

作　　者：邓雪阳[1,2] 李莉[1,2] 束盼[1,2] 吴刚[1,2] 秦民坚[1,2] 

机构地区：[1]中国药科大学中药资源学研究室 [2]中国药科大学教育部现代中药重点实验室,南京210009

出　　处：《中国天然药物》2010年第5期330-337,共8页

摘　　要：目的:试验设计(Statistical design of experiment)首次被运用于优化提取总酚酸过程。以优化超声提取千斤拔总酚酸为例, 研究此方法的适用性。方法:4 个提取因子, 乙醇浓度, 超声时间, 超声温度和溶剂溶质液固比用 full two-level factorial design 同时进行优化筛选; 根据筛选试验所得结果, 3 个显著因子, 乙醇浓度, 超声时间, 超声温度用face-centered composite design 进行响应面优化。结果:千斤拔优化超声提取总酚酸条件为:乙醇浓度 58.9 %, 超声时间 38 min, 超声温度 47.2 °C。在此条件下, 总酚含量为 40.36 mg·g-1, 它与预测值 39.88 mg·g-1 很好的吻合。结论:试验结果表明试验设计能够运用于优化千斤拔总酚提取过程。千斤拔是一种酚类含量较高的植物资源。AIM： Statistical design of experiment （DOE） was applied for the first time to optimize the extraction of phenolics, based on the study of the ultrasonic extraction of phenolics from Radix Flemingia philippinensis （RFP）. METHODS： Four extraction parameters, ethanol concentration, ultrasonic time, ultrasonic temperature and solvent to solid ratio, were simultaneously evaluated by a full two-level factorial design. On the basis of the screening design, three significant parameters, ethanol concentration, ultrasonic time, and the ultrasonic temperature, were finally optimized using a face-centered composite （FCCC） design. RESULTS： The results showed that the optimal ultrasonic conditions for RFP were ethanol concentrate of 58.9 %, ultrasonic time of 38 min, ultrasonic temperature of 47.2 ℃. Under such conditions, total phenolics were 40.36 mg GAE/g sample, which well matched the predicted value of 39.88 mg GAE/g sample. CONCLUSION： DOE can be successfully applied for extracting phenolics from RFP. The high phenolic content of RFP indicated that it could be a suitable natural phenolic resource.

关 键 词：试验设计 优化 超声提取 千斤拔 酚 

分 类 号：R284.2[医药卫生—中药学]