中药复方柴胡疏肝散体内外抗氧化活性及其与抗抑郁作用的 潜在关系（英文）  

In Vitro and in Vivo Antioxidant Effects and the Possible Relationship between the Antidepression Efficacy of Traditional Chinese Medicine Formulation Chaihu Shugan San

作　　者：李书启[1] 苏志恒[2] 彭静波[2] 邹忠梅[2] 喻长远[1] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029 [2]中国医学科学院北京协和医科大学药用植物研究所,北京100193

出　　处：《中国天然药物》2010年第5期353-361,共9页

基　　金：This project was supported by the Ministry of Education of the People’s Republic of China (No.20070023030);the National Natural Science Foundation of China (No. 30772711)~~

摘　　要：目的:研究中药复方柴胡疏肝散(CSGS)的体内外抗氧化活性, 探讨其抗氧化活性与抗抑郁作用之间的联系, 助以阐释CGGS抗抑郁的作用机制。方法:采用总抗氧化能力测定(FRAP法), DPPH (1 ,1-二苯基-2-苦基苯肼) 自由基(DPPH法)、ABTS[ 2 ,2-连氮(3-乙基苯并噻唑啉-6-磺酸)二铵盐]阳离子自由基(ABTS法)、超氧阴离子(O2·-)及羟自由基(·OH)清除能力测定5 种方法, 以维生素C为阳性对照, 研究CSGS体外抗氧化活性。建立束缚氧化应激模型, 通过测定全血中谷胱甘肽(GSH)含量, 肝组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MAD)含量研究CSGS体内抗氧化作用。结果:CSGS体外具有一定的铁离子还原能力和自由基清除能力, 其还原能力(FRAP法)每克CSGS提取物相当于 0.24 mmol FeSO4, 清除DPPH自由基、ABTS阳离子自由基、O2·-及 ?OH的IC50分别为 0.83, 1.03, 10.31 和 7.79 mg?mL-1。体内抗氧化活性研究显示, 在束缚应激状态下, CSGS给药组小鼠与模型组小鼠比较具有相对高的SOD和CAT活性及GSH含量, 相对较低的MDA水平。结论:复方柴胡疏肝散具有抑制脂质过氧化、缓解氧化应激损伤、调控机体抗氧化水平的药理作用, 其抗抑郁作用可能与其抗氧化活性有一定相关。AIM： To investigate the in vitro and in vivo antioxidant activities of the traditional Chinese prescription Chaihu Shugan San （CSGS）, which may help to understand the relationship between the antioxidant properties and the antidepressive effect of CSGS, to further clarify the possible mechanism of its antidepression efficacy. METHODS： The antioxidant activity in vitro was evaluated by employing five antioxidant assays including ferric reducing antioxidant power （FRAP） assay, DPPH （1, 1-diphenyl-2-picryl-hydrazyl） radicals, ABTS [2, 2＇-azino-bis （3-ethylbenzo-thiazoline-6-sulfonic acid） diammonium salt] cation radicals, superoxide anion （O2^· -） and hydroxyl free radicals （OH^·） scavenging activity assays. Vitamin C （Vc） was used as a positive control The in vivo study was carried out to investigate the effects of CSGS on mice in restraint stress model by determining glutathione （GSH） contents in blood and malondialdehyde （MDA） values, superoxide dismutase （SOD） and catalase （CAT） activities in liver. RESULTS： CSGS showed a ferric reducing antioxidant power （FRAP assay） that was equivalent to 0.24 mmol FeSO4 per gram CSGS aqueous extract and certain scavenging activities on free radicals in vitro. The IC50 values of CSGS in scavenging DPPH radicals, ABTS cation radicals, superoxide anion and hydroxyl free radicals were 0.83, 1.03, 10.31 and 7.79 mg·mL^-1, respectively. The results of in vivo study showed that the restrained mice with orally administered CSGS had higher SOD and catalase CAT activities, lower malondialdehyde MDA values and higher glutathione GSH levels compared with those of the mice in model group. CONCLUSION： CSGS could inhibit lipid peroxidation, relieve oxidative injury and regulate antioxidant capacity of organisms. The findings suggest that antioxidant activity of CSGS should make contributions to its antidepression effect.

关 键 词：柴胡疏肝散(CSGS) 抗氧化 抗抑郁 氧化应激 

分 类 号：R965[医药卫生—药理学]