DEL cDNA序列分析及蛋白质三维结构的模建  

Sequencing of DEL cDNA and homology comparative modeling for three-dimensional protein structure

作　　者：庄乃保[1] 张印则[1] 吴凡[1] 邵超鹏[1] 苏晓东[2] 

机构地区：[1]深圳市血液中心,深圳518035 [2]北京大学,北京100000

出　　处：《国际输血及血液学杂志》2010年第5期387-390,共4页International Journal of Blood Transfusion and Hematology

基　　金：国家自然科学基金课题（编号：30670893）

摘　　要：目的 分析DEL血型的cDNA序列,模拟并分析DEL血型蛋白质三维结构.方法 采用逆转录-聚合酶链反应（RT-PCR）技术和cDNA序列分析技术,得到DEL血型的cDNA序列并测序分析.通过同源比较模建（Homology comparative modeling）的思想,构建和分析正常阳性RHD和DEL9两种蛋白质的三维结构.结果 DEL血型个体有复杂的mRNA形式,共检测到6种形式的RhD转录子.DEL血型不存在正常的RhD mRNA.对正常RHD和与正常RHD序列相似度最高的DEL9蛋白质结构进行比较分析.结构显示DEL9蛋白属于α螺旋结构蛋白,其在膜外部分与正常RhD蛋白基本一致.结论 DEL血型可能具有正常RhD的抗原表位.Objective To analyze cDNA sequencing of DEL types and homology comparative modeling for three-dimensional protein structure. Methods DEL mRNAs were amplified through a reverse-transcriptase PCR （RT-PCR）, and then cDNAs were sequenced. A transcript of DEL9 was compared as normal RhD protein through homology comparative modeling for three-dimensional protein structure. Results Six kinds of transcripts were detected in the DEL individuals, but excluding the normal RhD isolation. The most similar one, DEL9 transcript, however, showed the same α-helix structure with the normal RhD protein, especially its extramembranous parts seemed absolutely consistent with the normal one. Conclusion DEL may have complete D antigen epitopes as the ones on normal D-positive red blood cells.

关 键 词：RH血型 抗-D阴性 DEL CDNA 转录子 蛋白质三维结构 

分 类 号：R4[医药卫生—临床医学]