吡那地尔对人肺动脉平滑肌细胞内Ca^(2+)浓度的影响  

Effects of pinacidil on intracellular free calcium concentration of primary cultured human pulmonary artery smooth muscle cells

作　　者：朱煜明[1] 解卫平[2] 王虹[2] 

机构地区：[1]常州市第一人民医院呼吸病科,213001 [2]南京医科大学第一附属医院呼吸病科

出　　处：《江苏医药》2010年第18期2113-2115,共3页Jiangsu Medical Journal

基　　金：江苏省自然科学基金资助项目(BK2006246)

摘　　要：目的探讨ATP敏感性钾(KATP)通道开放剂吡那地尔(Pin)对内皮素1(ET-1)诱导的人肺动脉平滑肌细胞(PASMCs)增殖及细胞内[Ca2+]i的影响。方法体外培养人PASMCs,用ET-1诱导其增殖,应用MTT法、Fluo-3和激光扫描共聚焦显微镜技术评价Pin对ET-1诱导的人PASMCs增殖及PASMCs[Ca2+]i调节的作用。结果 Pin显著抑制ET-1诱导的人PASMCs增殖,呈浓度依赖效应,KATP通道拮抗剂格列本脲呈浓度依赖性阻断Pin的作用;ET-1诱导人PASMCs内[Ca2+]i显著增加,Pin(10μmol/L)拮抗ET-1诱导的人PASMCs内[Ca2+]i升高。结论 KATP通道开放剂Pin可明显抑制ET-1诱导的人PASMCs增殖作用,抑制细胞内Ca2+浓度增加。Objective To study the effects of pinacidil on the proliferation and intracellular free calcium concentration of primary cultured human pulmonary artery smooth muscle cells （PASMCs） induced by endothelin-1 （ET-1）. Methods The experimental models of proliferation of primary cultured human PASMCs induced by ET-1 in vitro were established.The influence of pinacidil on vitality of PASMCs was detected by MTT,and intracellular free calcium concentration （／[Ca^2＋／]i） was observed by the fluorescence Ca^2＋ indicator Fluo-3/AM. Results Pinacidil inhibited the proliferation of human PASMCs in a concentration-dependent manner.Glibenclimide,a selective ATP-sensitive K＋ （KATP） channel blocker,abolished the effect of pinacidil on the cell proliferation in a concentration-dependent manner. The treatment with ET-1 resulted in a transient raise of ／[Ca^2＋／]i in human PASMCs,which could be blocked by pinacidil 10 μmol/L.Conclusion Pinacidil inhibits the proliferation of human PASMCs and decreases intracellular free calcium concentration of cultured human PASMCs induced by ET-1.

关 键 词：ATP敏感性钾通道 人肺动脉平滑肌细胞 钙通道 

分 类 号：R563[医药卫生—呼吸系统]