NF-κB诱捕ODNs对子宫内膜息肉间质细胞环氧化酶及雌激素生成的影响  

Effect of NF-κB decoy ODNs on the synthesis of COX-2 and estradiol in stromal cells of endometrial polyps

作　　者：纪统慧[1] 王秀丽[1] 戴辉华[1] 瞿琳[1] 

机构地区：[1]南京医科大学第一附属医院妇产科,210029

出　　处：《江苏医药》2010年第18期2119-2122,共4页Jiangsu Medical Journal

基　　金：国家自然科学基金(30600671);江苏省自然科学基金(BK2006577)

摘　　要：目的探讨核因子κB(NF-κB)诱捕脱氧寡核苷酸(ODNs)对子宫内膜息肉(EP)间质细胞在白细胞介素1β(IL-1β)诱导培养下环氧化酶2(COX-2)合成及雌激素(E2)分泌的影响。方法 15例EP患者行间质细胞的分离培养,分为3组:刺激(A)组,用10μg/L的IL-1β刺激培养;转染后刺激(B)组,NF-κB诱捕ODNs转染EP间质细胞后,用10μg/LIL-1β刺激培养;空白对照(C)组。培养12、24、36和48h,RT-PCR检测细胞中COX-2水平,ELISA检测细胞培养上清中E2水平。结果 A组自12h开始COX-2合成显著高于C组,自24h开始E2的分泌量显著高于C组(P<0.01)。B组COX-2合成及E2分泌较A组显著下降(P<0.01)。结论 NF-κB诱捕ODNs能显著抑制IL-1β诱导的EP间质细胞合成COX-2及分泌E2。Objective To explore the effect of neuclear factor（NF）-κB decoy ODNs on the synthesis of cyclooxegenase（COX）-2 and estradiol（E2） in stromal cells of endometrial polyps（EP） induced by interleukin（IL）-1β.Methods Stromal cells from 15 EP cases were purified,cultured and divided into 3 groups with 5 cases each.Group A were cultured with 10 μg/L of IL-1β,group B was transfected by NF-κB decoy ODNs and then cultured with 10 μg/L of IL-1β.Group C was taken as blank control.At 12,24,36 and 48 h,the expression of COX-2 in stromal cells was detected by RT-PCR and the concentration of E2 in the supernatant was measured by ELISA.Results The expression of COX-2 since 12 h and E2 secretion since 24 h were significantly higher in group A than those in group C（P0.01）,which were significantly lower in group B than those in group A（P0.01）.Conclusion NF-κB decoy ODNs can significantly decrease the COX-2 expression and E2 secretion stimulated by IL-1β in stromal cells of EP.

关 键 词：环氧化酶2 子宫内膜息肉 核因子ΚB 雌激素 

分 类 号：R711[医药卫生—妇产科学]