四川棉花立枯丝核菌(Rhizoctonia solani Kühn)菌丝融合群研究  

A study on the anostomasis group of Rhizoctonia solaniKühn in Sichuan Province

作　　者：陈素清[1,2,3] 吴斌[1,2,3] 秦蓁[1,2,3] 刘光珍 邓丽 邓先明 

机构地区：[1]四川省农业厅植检站 [2]四川省农科院植保所 [3]西南农业大学

出　　处：《西南农业学报》1999年第1期56-60,共5页Southwest China Journal of Agricultural Sciences

摘　　要：从四川棉区采集棉花立枯病标样，分离得到６９９个分离物，从中鉴定出４１４个丝核菌（Ｒｈｉｚｏｔｏｎｉａｓｐｐ）菌株，经Ｇｉｅｍｓａ核相染色，鉴定出４０９个菌株属多核丝核菌（ＭｕｌｔｉｎｕｃｌｅａｔｅＲｈｉｚｏｃｔｏｎｉａｓｐｐ），占丝核菌总数的９８．８％。另５个菌株属双核丝核菌，占总数的１．２％。用载片定位融合法，以生越明（１９９２）提供的国际标准菌测定，４０９个多核丝核菌分属三个菌丝融合群，即ＡＧ－１、ＡＧ－２和ＡＧ－４，其中ＡＧ－４出现比率占多核丝核菌总数的７９％，是四川棉苗立枯丝核菌的代表菌。室内、外致病力测定结果，以ＡＧ－４融合群对棉花的致病力最强，ＡＧ－１融合群居中，ＡＧ－２融合群最弱，三者室内与田间平均病指数分别为９５．９、５７．６和３６．２。699 isolates were isolated from cotton drooping disease samples which w ere collected from Sichuan cotton productive areas,and 414 Rhizoctonia s pp.strains were identified from them.Through Giemsa staining,it was identified t hat 409 strains belong to multinucleate Rhizoctonia spp,acounting for 98.8% of the total Rhizoctonia spp;5 belong to binucleate Rhizoctonia spp,whic h was 1.2% of the total Rhizoctonia spp. Results from cover glass fixed pos ition mating method and the tester isolates provided by Namakoshi Ake showed th at 409 multinucleate Rhizoctonia spp might be divided into 3 anostomosis gro ups, AG-1,AG-2 and AG-4.The frequency of AG-4 was 79% of the multinucleate Rhizoctonia,which was the representativ e st rain of Rhizoctonia solani in Sichuan Province.The testing results of pathog enic ity in lab and field showed that AG-4 had the strongest pathogenicity,and its me an disease index was 95.9;Whereas AG-2 had the weakest pathogenicity,its mean di sease index being 362;AG-1 was medium,its mean disease index being 576.

关 键 词：棉花立枯丝核菌 菌丝融合群 致病力测定 四川 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治]