转化生长因子β_2在发育性髋关节发育不良患儿圆韧带中的分布和表达  

Distribution and expression of transforming growth factor beta 2 in the ligamentum teres of children with developmental dislocation of the hip

作　　者：史立伟[1] 赵群[1] 张立军[1] 李连永[1] 高红[2] 

机构地区：[1]中国医科大学附属盛京医院小儿外科,辽宁省沈阳市110004 [2]中国医科大学附属盛京医院卫生部小儿先天畸形重点实验室,辽宁省沈阳市110004

出　　处：《中国组织工程研究与临床康复》2010年第37期6865-6868,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金项目(30600654);课题名称:17q21区域内发育性髋关节脱位易感基因的克隆;鉴定及功能研究~~

摘　　要：背景:发育性髋关节发育不良患儿关节松弛,很可能与细胞外基质成分有关。转化生长因子β2调控Ⅰ型胶原基因表达的关键性细胞因子,能促进成纤维细胞的生长、分裂,并能分泌胶原蛋白,是骨科学中纤维异常相关研究的方向之一。目的:观察发育性髋关节脱位患儿和正常儿转化生长因子β2在圆韧带中分布规律与其mRNA及蛋白质表达的差异,探索髋关节松弛的原因。方法:选取性别相同、年龄相近的6对发育性髋关节脱位患者与正常儿进行配对比较,采用免疫组化和半定量RT-PCR技术检测圆韧带中转化生长因子β2及其mRNA的分布规律与表达差异。结果与结论:分泌转化生长因子β2的成纤维细胞贴近圆韧带关节侧的滑膜层,内部纤维层转化生长因子β2阳性表达细胞稀疏。发育性髋关节脱位患儿纤维层中表达转化生长因子β2的成纤维细胞数数量明显减少(P<0.01),圆韧带中转化生长因子β2mRNA表达也明显减少(P<0.01)。由此推测,圆韧带中转化生长因子β2的分布减少和表达异常很可能与发育性髋关节发育不良患儿的髋关节松弛有关。BACKGROUND：Joint laxity in children with developmental dislocation of the hip（DDH） may relate to the component of extracellular matrix.Transforming growth factor-β2（TGF-β2） regulates the expression of type Ⅰ collagen and can promote cell growth,division and secrete collagen protein,this is a direction for study of fibrous dysplasia in orthopaedics and tranmatology.OBJECTIVE：To observe distribution and expression discrepancy of TGF-β2 in the ligamentum teres of children with DDH and normal children,additionally,to investigate the roles of TGF-β2 in hip joint laxity.METHODS：Six children with DDH and six age and gender matched normal children were enrolled.Immunohistochemistry and semiquantitative RT-PCR were used to detect the distribution and mRNA expression of the TGF-β2 in the ligamentum teres.RESULTS AND CONCLUSION：Fibroblasts secreted TGF-β2 was observed in the synovial layer that regularly arranged parallel to the joint surface,and there were few positive-TGF-β2 in the internal fibrous layer of the ligamentum teres.Compared with the normal children,the number of positive fibroblasts was obvious decreased in children with DDH（P〈0.01）,and the TGF-β2 expression was decreased at mRNA level（P〈0.01）.Accordingly,the decreased and abnormal expression of TGF-β2 in the ligamentum teres probably related to hip joint laxity in children with DDH.

关 键 词：髋关节发育不良 关节松弛 圆韧带 转化生长因子Β2 组织工程 

分 类 号：R681.1[医药卫生—骨科学]