复方茶多酚软膏对放射性皮炎大鼠伤口愈合和表皮生长因子表达的影响  

Effects of compound tea polyphenol ointment on wound healing and epidermal growth factor expression of radiation-induced cervical skin dermatitis in rats

作　　者：薛俐[1] 冯志明[2] 易银沙[2] 阎政礼[2] 李旭红[1] 

机构地区：[1]中南大学湘雅三医院,湖南省长沙市410013 [2]湖南师范大学制药工程系,湖南省长沙市410081

出　　处：《中国组织工程研究与临床康复》2010年第37期6891-6894,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：湖南省中医药科研计划资助项目(No2008096);题目:复方茶多酚对放射性皮肤溃疡的的防治作用;湖南省科技计划资助项目(No2009SK3171);题目:慢病毒介导CTGF-shRNA对放疗后皮肤成纤维细胞CTGF-mRNA的影响~~

摘　　要：背景:研究表明复方茶多酚软膏对急性放射性皮炎的伤口有愈合作用,但具体机制尚未阐明。目的:探讨复方茶多酚软膏对大鼠放射性皮炎创面愈合及表皮生长因子表达的影响。方法:清洁级雄性成年Wistar大鼠随机分成3组:实验组和对照组大鼠通过60Coγ射线照射建立大鼠放射性皮炎模型,实验组大鼠采用常规护理后用复方茶多酚软膏外涂辐射伤口区,对照组涂抹凡士林软膏;简单皮肤伤口组大鼠采用1.5cm2皮肤全切除,仅接受常规护理。通过放射免疫法测定伤后5,10,20和40d时创面组织的表皮生长因子表达、观测创面愈合率和愈合时间,并与简单伤口组比较。结果与结论:实验组和对照组在γ射线照射后5d创面组织表皮生长因子表达弱于简单皮肤伤口组,提示放射性皮炎与简单皮肤切口损失不同,创面恢复期创面组织表皮生长因子呈弱表达,愈合缓慢;实验组创伤后10和20d时创面组织表皮生长因子表达显著高于对照组(P<0.05);实验组在10和20d时的创面愈合率明显高于对照组(P<0.05);实验组愈合时间明显短于对照组的愈合时间(P<0.05),实验组和对照组的愈合时间均明显长于简单皮肤伤口组(P<0.05)。结果表明复方茶多酚软膏外涂对大鼠放射性皮炎的愈合有促进作用,其机制可能与促进创面组织表皮生长因子的表达有关。BACKGROUND：Studies demonstrated that compound tea polyphenol ointment promotes wound healing in radiation-induced cervical skin dermatitis,but the precise mechanisms remain poorly understood.OBJECTIVE：To study the effects of compound tea polyphenol ointment on wound healing rate and epidermal growth factor（EGF） expression of radiation-induced dermatitis in rats.METHODS：Adult Wistar rats were randomly divided into 3 groups.Rats in the experimental and control groups were established 60Coγ radiation-induced dermatitis models and treated by compound tea polyphenol ointment or Vaseline,respectively.In the simple wound group,1.5-cm2 skin of rats were removed and treated by routine nursing care.The EGF expression in wound tissue was analyzed by radioimmunoassay,and the wound healing rate and wound healing time was also measured at 5,10,20,30 and 40 days after radiation.RESULTS AND CONCLUSION：The EGF expressions of wound skin tissues in the experiment and control groups were higher than that of the simple wound group at 5 days after radiation,which revealed that the radiodermatitis was different from simple wound and healed slowly.Compared with the control group,the EGF expression of wound skin tissues in the experiment group was significantly higher（P〈0.05） and the wound healing rate was significantly greater at 10 and 20 days after radiation（P〈0.05）.The wound healing time of the experiment groups was significantly shorter than that of the control group（P〈0.05）.But the wound healing time of the experiment and control groups was longer that that of the simple wound group（P〈0.05）.Compound tea polyphenol ointment can significantly promote the wound healing of radiation-induced dermatitis in rats,which may be related to accelerating the EGF expression of wound skin tissues.

关 键 词：放射性皮炎 大鼠 茶多酚 黄芩苷 表皮生长因子 

分 类 号：R318[医药卫生—生物医学工程]