模拟人后外侧入路髓核摘除构建的椎间盘退行性变动物模型  

Constructing a rabbit model of intervertebral disc degeneration induced by puncture and aspiration through a posterolateral approach

作　　者：陈国仙 王万明[2] 林宗锦 李国山 曾清东 林智军[3] 刘航涛[3] 

机构地区：[1]莆田市第一医院骨科,福建省莆田市351100 [2]解放军南京军区福州总医院骨二科,福建省福州市350025 [3]解放军南京军区福州总医院附属第一医院骨科,福建省莆田市351100

出　　处：《中国组织工程研究与临床康复》2010年第37期6904-6907,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:椎间盘退变的病理生理学机制至今尚未明了,所以制作一种与自然椎间盘退行性病变过程相类似的、适用于这些治疗方法的动物模型有助于对椎间盘退行性变的诱因、病理生理变化及某些治疗方法的研究。目的:模拟人后路髓核摘除术进行后外侧穿刺抽吸髓核建立椎间盘退行性变动物模型的可行性研究及其优点。方法:随机选取日本大耳白兔20只,根据解剖结构观察结果,为防止纤维环后外侧穿刺损伤血管,采用右后外侧入路,行L1～2和L3～4椎间盘后外侧穿刺髓核抽吸法摘除部分髓核组织,将L2～3椎间盘纳入对照组。术后2,4,8,12周分别对造模后椎间盘及对照椎间盘(L2～3)行X射线及MRI影像学观察。结果与结论:日本大耳白兔20只均进入结果分析,术后2,4,8,12周实验组椎间盘高度指数持续下降(P<0.05),说明造模后椎间盘间隙高度会逐渐降低,并且各时间点椎间盘高度指数明显低于对照组(P<0.05)。与对照组比较,造模后2,4,8,12周时实验组椎间盘MRIT2WI信号逐渐下降,并且随时间延长逐渐降低,呈低信号改变。通过影像学观察发现本模型退行性变的征象与人腰椎间盘退行性变的征象一致,提示模拟人后路髓核摘除术建立后外侧纤维环穿刺髓核抽吸的椎间盘退行性变动物模型成功建立,为运用组织工程修复重建退行性变椎间盘的研究提供有效的动物模型。BACKGROUND：Pathophysiology mechanism involved in disc degeneration remains poorly understood,thus,construction of an animal model that similar to natural intervertebral disc degeneration is benefit for the study of pathological and physiological changes of disc degeneration.OBJECTIVE：To establish a rabbit model similar to simulate human discectomy intervertebral disc degeneration induced by puncture and aspiration,and to analyze its feasibility and advantage.METHODS：A total of 20 Japanese white rabbits were randomly selected,according to anatomical observations,in order to prevent puncture injuries posterolateral annular vessel,the right posterior lateral approach was used,parts of nucleus pulposus of the line L1-2 and L3-4 disc was removed by puncturing the posterior lateral nucleus liposuction,and the L2-3 intervertebral disc was served as the control group.X-ray and MRI imaging observation were performed on the disc intervertebral disc（L2-3） line at 2,4,8,and 12 weeks after modeling.RESULTS AND CONCLUSION：All of 20 rabbits were involved in the result analysis.The intervertebral disc height index of the experimental group continued to decline at 2,4,8 and 12 weeks after operation（P 0.05）,it illustrated that the height of intervertebral disc would be gradually reduced after modeling,and the disc height index was significantly lower than the control group at each time point（P 0.05）.Compared with the control group,MRI T2WI signal of the experimental group disc gradually decreased at 2,4,8 and 12 weeks after operation,and gradually decreased with time prolonged,which showed a low signal change.The imaging observation demonstrated that lumbar intervertebral disc degeneration of animal model exhibited a similar tendency to humans,suggesting that a rabbit model of intervertebral disc degeneration induced by puncture and aspiration through a posterolateral approach was successfully established,which provides an effective animal model for the repair and reconstruction using tissue-engineered interver

关 键 词：椎间盘退行性病变 动物模型 针刺抽吸法 造模实验 组织构建 

分 类 号：R318[医药卫生—生物医学工程]