壮骨镇痛胶囊含药血浆培养乳腺癌细胞rac-1,PI3-K转录和细胞伪足的变化  

Effects of plasma containing zhuanggu zhentong capsules on the transcription of rac-1,PI3-K and pseudopodia of breast cancer cell lines in vitro

作　　者：柳景红[1] 曹建雄[1] 何迎春[2] 田道法 卢芳国[2] 卓耀[1] 

机构地区：[1]湖南中医药大学附属第一医院肿瘤科,湖南省长沙市410007 [2]湖南中医药大学中西医结合学院,湖南省长沙市410007 [3]湖南省中医五官重点学科,湖南省长沙市410007

出　　处：《中国组织工程研究与临床康复》2010年第37期6951-6954,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：湖南省科技厅项目(2007TP4021);名称:壮骨镇痛胶囊抗裸鼠乳腺癌骨转移瘤机理实验研究;湖南省科技厅项目(湘财教指2009-42);名称:壮骨镇痛胶囊抗恶性肿瘤骨转移机制的研究;湖南省教育厅项目(08C637);名称:壮骨镇痛散抗裸鼠乳腺癌骨转移瘤及对肿瘤微血管形成影响的实验研究;湖南省教育厅项目(09C724);名称:壮骨镇痛胶囊抗乳腺癌骨转移分子机制的研究~~

摘　　要：背景:前期实验已证实壮骨镇痛胶囊能显著抑制体外培养乳腺癌细胞的迁移和增殖。目的:进一步观察壮骨镇痛胶囊对高转移人乳腺癌MDA-MB-231细胞rac-1,PI3-K转录和细胞伪足生长的影响。方法:SD大鼠以3倍剂量灌服壮骨镇痛胶囊连续7d,以大鼠含药血浆培养MDA-MB-231细胞24h后,采用扫描电镜法观察不同浓度壮骨镇痛胶囊含药血浆对MDA-MB-231细胞伪足生长的影响,采用RT-PCR法检测含药血浆对MDA-MB-231细胞中rac-1,PI3-K转录的影响。结果与结论:5.0%和1.25%壮骨镇痛胶囊含药血浆能显著抑制MDA-MB-231细胞伪足的形成和生长以及rac-1和PI3-K基因的转录(P<0.05);0.63%壮骨镇痛胶囊含药血浆对伪足生长和rac-1基因转录无显著影响,但可显著抑制了PI3-K基因的转录(P<0.05)。由此推测壮骨镇痛胶囊含药血浆抑制MDA-MB-231细胞迁移可能与其抑制rac-1和PI3-K基因转录进而影响细胞伪足形成和生长密切有关,并且该作用可能与浓度相关。BACKGROUND：Previous studies have demonstrated that zhuanggu zhentong capsules（ZZC） can significantly inhibit the migrations and proliferations of breast cancer cell lines in vitro.OBJECTIVE：Based on the previous experiment,to study the effects of ZZC on the transcriptions of rac-1 and PI3-K and pseudopodia of breast cancer cell lines MDA-MB-231 in vitro.METHODS：SD rats were drenched 3-fold doses ZZC for 7 successively days,and then rat blood plasma were obtained to culture MDA-MB-231 cells for 24 hours,after that,the effects of drug blood plasma on pseudopodia of MDA-MB-231 cells and transcription of migrating relative genes rac-1 and PI3-K were observed under a scanning electronic microscope and Reverse transcriptase PCR（RT-PCR）.RESULTS AND CONCLUSION：The transcriptions of rac-1 and PI3-K genes and the development of pseudopodia of MDA-MB-231 cells were obviously inhibited when treated with the ZZC contained plasma at 5% and 1.25% concentration（P 0.05）.ZZC contained plasma at 0.63% concentration had no obvious inhibition on the transcriptions of rac-1 gene and the development of pseudopodia,but it obviously inhibited the transcriptions of PI3-K gene of MDA-MB-231 cells.It supposed that the inhibitive effects of ZZC contained plasma on MDA-MB-231 cells migrations in dose-dependent manner may be related to its inhibiting effects on the transcriptions of rac-1 and PI3-K genes and the development of pseudopodia.

关 键 词：壮骨镇痛胶囊 含药血浆 乳腺癌细胞 rac-1 PI3-K 细胞伪足 

分 类 号：R318[医药卫生—生物医学工程]