雌激素影响去势雌兔腰椎间盘髓核中白细胞介素20和基质金属蛋白酶3的表达?  

Whether estrogen affects the expression of interleukin-20 and matrix metalloproteinase-3 in lumbar nucleus gelatinosus of ovariectomized rabbits?

作　　者：白晓亮[1] 陈亮[1] 陈春茂[1] 

机构地区：[1]苏州大学附属第一医院骨科,江苏省苏州市215006

出　　处：《中国组织工程研究与临床康复》2010年第37期6955-6958,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:雌激素缺乏可导致椎间盘发生一系列的物理化学变化,有研究报道基质金属蛋白酶3可降解蛋白多糖聚合体及椎间盘内多种基质成分,白细胞介素20在椎间盘发生退变的过程中也发挥着重要作用。目的:探讨雌激素对去势雌兔腰椎间盘髓核中白细胞介素20和基质金属蛋白酶3表达的作用。方法:将新西兰大白兔随机分为正常对照组、模型组、低剂量和高剂量雌激素组。除正常对照组外均切除双侧卵巢建立去势动物模型。正常对照组和模型组隔日肌注生理盐水2mL,低、高剂量雌激素组分别隔日肌注0.2和1.0mg的苯甲酸雌二醇。3个月后所有动物于腰椎间盘髓核组织取材,采用苏木精-伊红染色、免疫组织化学染色和Western-blot方法检测白细胞介素20和基质金属蛋白酶3的表达。结果与结论:模型组细胞密度明显降低,脊索细胞明显减少,软骨样细胞集簇成团,其余各组无明显变化。免疫组化结果显示模型组腰椎间盘髓核组织白细胞介素20和基质金属蛋白酶3阳性细胞表达明显高于正常对照组和高剂量雌激素组(P<0.05)。Western-blot检测结果与免疫组化学染色结果相似。提示肌注雌激素能降低去卵巢新西兰大白兔腰椎间盘髓核中白细胞介素20和基质金属蛋白酶3的表达,进而防止腰椎间盘髓核基质的降解。BACKGROUND：Estrogen deficiency can result in a series of physical or chemical changes in intervertebral disc.Studies have demonstrated that matrix metalloproteinase-3（MMP-3） can degrade proteoglycan poly and ground substance of intervertebral disc.Interleukin-20（IL-20） also plays an important part in intervertebral disc degeneration.OBJECTIVE：To explore the effect of estrogen on IL-20 and MMP-3 expression in lumbar nucleus gelatinosus of ovariectomized rabbits.METHODS：New Zealand white rabbits were randomly divided into the normal control,model,low-dose estrogen and high-dose estrogen groups.All rabbits were prepared for ovariectomized models except that of the normal control group.Sodium chloride（2 mL）,estradiol benzoate（0.2 mg or 1 mg） were intramuscular injected into animals in the normal control,low-and high-dose estrogen groups every other day,respectively.The lumbar nucleus was harvested at 3 months after operation.The expression of IL-20 and MMP-3 was tested by hematoxylin-eosin staining,immumohistochemistry and Western-blot.RESULTS AND CONCLUSION：The cell density and notochord cells were obviously decreased in the model group,with agglomerate notochord-like cells.There were no marked changes in the other groups.The immumohistochemistry results demonstrated that,the positive expression of IL-20 and MMP-3 in the model group was greater that of the normal control and high-dose estrogen group（P 0.05）.Western-blot exhibited similar results as immumohistochemistry.The results demonstrated that intramuscular injection of estrogen can decrease expression of IL-20 and MMP-3 in degenerated lumbar nucleus,thereby,prevent degradation of nucleus pulposus.

关 键 词：腰椎间盘髓核 雌激素 基质金属蛋白酶3 白细胞介素20 组织构建 

分 类 号：R318[医药卫生—生物医学工程]