大网膜与颈部皮下包埋DegraPol支架促进体内再血管化的比较  

Comparison on promoting revascularization of DegraPol scaffold covered by omentum and subcutaneous in vivo

作　　者：杨林 武延格 孙学峰 王正 

机构地区：[1]暨南大学第二临床医学院深圳市人民医院胸外科,广东省深圳市518020

出　　处：《中国组织工程研究与临床康复》2010年第38期7048-7050,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30500499)课题"大网膜包埋促进血管再生;不同发展程度的组织工程气管原位重建的动物实验研究"~~

摘　　要：背景:如何解决工程化器官植入体内时快速血管化问题是组织工程取得成功的关键问题。目的:比较大网膜与颈部皮下两种方法包埋促进组织工程支架材料DegraPol血管化的差异,为工程化组织体内快速再血管化寻找有效方法。方法:应用解剖学中的血管铸型技术,比较DegraPol支架置入大鼠颈部皮下和腹部大网膜包埋新生血管形成的不同。结果与结论:DegraPol支架外部发现大网膜包埋组比颈部皮下包埋组有更多的蓝色显影微细血管环绕,在微孔支架内部,也有相对丰富的新生血管形成。颈部皮下包埋组在微孔支架内部未发现新生血管形成。大网膜包埋组再血管化程度高于颈部皮下组,差异有显著性意义(P<0.05)。提示大网膜包埋有效地促进血液循环建立,可作为组织工程化组织再血管化的可行方法。BACKGROUND：How to solve rapid vascularization is the key to success of tissue engineering when the tissue engineered organs are implanted into body.OBJECTIVE：To compare the difference of revascularization between the tissue engineered scaffold DegraPol covered by omentum and subcutaneous,and to find an effective method for rapid revascularization in vivo.METHODS：Using the anatomic technology of vascular cast,revascularization of DegraPol scaffold implanted by omentum and subcutaneously was compared.RESULTS AND CONCLUSION：More blue developing micro-capillary vessels wrapped the outer DegraPol scaffolds in omental implants than in subcutaneous implants.In porous scaffold,relative abundant new vessels formed in omental implant group.Subcutaneous implant group found no neovacularization.The vascularization degree of omentum implant was significantly higher than subcutaneous implant （P 0.05）.Omentum is effective in promoting to establish blood circulation,can be used as a viable method of tissue engineered revascularization.

关 键 词：再血管化 组织工程 血管铸型 支架 材料 

分 类 号：R318[医药卫生—生物医学工程]