帕金森病模型大鼠行为学改变与黒质致密部多巴胺能神经元存活率之间的相关性  

Correlation of Behavioral Changes and Survival Rate of Dopaminergic Neurons in the Substantia Nigra Pars Compacta in Parkinsonian Rat Model

作　　者：刘丽敏[1] 李博[1] 孙作厘[1] 贾军[1] 汪璇[1] 王晓民[1] 

机构地区：[1]首都医科大学基础医学院生理学系,教育部神经变性病重点实验室,北京100069

出　　处：《中国实验动物学报》2010年第5期394-397,I0005,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(编号:30472245);首都医科大学基础临床科研合作基金(编号:10JL14)

摘　　要：目的探讨线刀损毁内侧前脑束建立的帕金森病模型大鼠行为学改变与黒质致密部多巴胺能神经元存活率之间的相关性。方法采用可伸缩线刀切断大鼠内侧前脑束建立单侧损伤的帕金森病大鼠模型;皮下注射阿朴吗啡后测试大鼠的旋转行为;取中脑黑质切片进行酪氨酸羟化酶免疫组织化学染色,通过计数黑质致密部酪氨酸羟化酶阳性神经元的数目得到多巴胺能神经元存活率。结果模型组大鼠损伤侧酪氨酸羟化酶阳性神经元的数目明显下降,而由阿朴吗啡诱导的旋转行为明显增加。结论线刀损毁术后4周,帕金森病模型大鼠由阿朴吗啡诱导的旋转行为和黒质致密部多巴胺能神经元存活率之间存在着明显的负相关。Objective To explore the correlation of behavioral changes and survival rate of dopaminergic neurons in the substantia nigra pars compacta （SNpc） in parkinsonian rat model.Methods The parkinsonian rat model was established by mechanical transection of the nigrostriatal dopamine pathway at the medial forebrain bundle （MFB）.Behavioral studies were carried out by apomorphine challenge.Immunohistochemistry was applied on the tissue sections of substantia nigra （SN）.The number of dopaminergic neurons was determined by counting the tyrosine hydroxylase （TH）positive neurons in the sections.Results Immunohistochemical studies confirmed a significant loss of TH-positive neurons on the lesioned side,while the rotations induced by apomorphine increased remarkably in the parkinsonian rat model.Conclusions At 4 weeks after the surgery,there is a significant inverse correlation between the number of apomorphineinduced rotations and the decreased survival rate of neurons in parkinsonian rat models established by mechanical transection of the nigrostriatal dopamine pathway at the medial forebrain bundle.

关 键 词：帕金森病 内侧前脑束 阿朴吗啡 多巴胺能神经元 

分 类 号：Q-189[生物学]