苏木单体物对大鼠心脏移植后NF-κB信号传导途径的影响  

Effect of Caesalpinia sappan on NF-κB signal transduction pathway after cardiac transplantation in rats

作　　者：邹永鹏[1] 吴健[1] 张毛毛[1] 张奇[1] 侯静波[1] 于波[1] 

机构地区：[1]哈尔滨医科大学第二临床医学院心内科教育部黑龙江省部共建心肌缺血机理与诊疗技术重点实验室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2010年第5期417-419,共3页Journal of Harbin Medical University

基　　金：中国博士后基金资助项目(63177);黑龙江省自然科学基金项目(ZJY-3-7);教育部博士点新教师类资助项目(20092307120009);黑龙江省科学技术计划项目(GC02C122)

摘　　要：目的探讨苏木中药对移植心脏的保护作用及可能的作用机制。方法建立大鼠腹腔同种异位心脏移植模型。实验分为3组:A组为空白对照组;B组为苏木(Caesalpinia sappan,CS)中药组;C组为环孢素(CsA)组。每组8只大鼠。术后第2天开始灌药,第7天取供心。Western blot法检测心肌组织中NF-κB及IκBα的表达水平。结果与移植对照组相比CsA及CS组移植物的病理损害明显减轻;NF-κB及IκBα的表达水平给药组较对照组差异显著(P<0.05),但两种药物组之间的差异不显著(P>0.05)。结论苏木中药可能通过抑制NF-κB信号传导途径的激活,减缓急性排斥反应的发生从而减轻移植物的病理损伤,发挥保护移植心脏的作用。Objective To discuss the protective effect and possible mechanism of Caesalpinia sappan on the transplanted heart.Methods The model of cardiac allotransplantation was established.The cardic allograft rats were randomly divided into 3 groups： control group（A group）,Caesalpinia sappan group（B group）,and cyclosporin A group（C group）.Animals were given drugs 2 days after operation.The expression of NF-κB P65 and IκBα were measured 7 days after transplantation by Western blot.Results The allogeneic graft survival time were significantly prolonged in CsA and CS groups.The level of NF-κB P65 and IκBα in CsA and CS group had a significant difference compared with control group（P0.05）,but the difference between the two medicines was not significant（P0.05）.Conclusion Caesalpinia sappan may inhibit the activation of NF-κB signal transduction pathway,thereby slow down the acute rejection and reduce the pathological injury of transplanted heart.

关 键 词：同种异体移植 排斥反应 心脏移植 NF-κB IΚBΑ 

分 类 号：R654.2[医药卫生—外科学]