胸腺内注射不同剂量的异基因抗原诱导大鼠同种异体神经移植的免疫耐受反应  

Immunotolerance reaction for allograft nerve in rat induced by intrathymic injection with different dose of allogenic antigen

作　　者：周辰亮[1] 孟庆刚[1] 张凤蕴[2] 钱贵宾[1] 孙丕云[1] 李卫[1] 

机构地区：[1]哈尔滨医科大学第四临床医学院骨科,黑龙江哈尔滨150001 [2]哈尔滨医科大学免疫学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2010年第5期424-427,F0004,共5页Journal of Harbin Medical University

基　　金：黑龙江省科技厅基金资助项目(D2004-46)

摘　　要：目的探讨大鼠胸腺内注射不同剂量异基因抗原(MHC),诱导大鼠同种异体坐骨神经产生的特异性免疫耐受反应。方法雄性Wistar大鼠20只为供体,选用清洁级雄性SD大鼠50只为受体,受体随机分为5组,每组10只:A:新鲜自体神经移植组;B:新鲜异体神经移植组;C、D、E组分别为低、中、高剂量异基因抗原注射异体神经移植组,剂量分别为1.5 mg、3.0 mg、6.0 mg。术后2周进行运动神经传导速度、病理学、混合淋巴细胞培养(MLC)和血清sIL-2R检查。结果胸腺内抗原注射组动物肢体的生理功能、免疫学、组织学及透射电镜指标恢复,其中,E组动物各项指标恢复最明显,与A组指标类似。结论胸腺内注射异基因抗原可以诱导大鼠对异体坐骨神经移植的免疫耐受,在一定范围内,免疫耐受的程度与抗原量有关。Objective To investigate the influence of intrathymic injection with different dose of allogenic antigen in induction of sciatic allograft immunotolerance in rats.Methods Twenty male Wistar rats were used as transplant donors,male Sprague Dawley（SD） rats were divided into 5 groups（10 rats in each group）： nerve autograft group,nerve allograft group,low-dose,moderate-dose and high-dose MHC antigen injection groups.The dose of MHC antigen per group were 1.5 mg,3 mg,6 mg.Two weeks after the surgery,nerve electrophysiology,pathology,sIL-2R,mixed lymphocyte culture and delayed type hypersensitivity were examined.Results Pretreated animals receiving intrathymic injection recovered limb function,and demonstrated by Motor nerve conduction velocity,immunological paremeters,nerve graft histology and electromicroscope.The paremeters in group E were similar to those measured in group A.Conclusion Pretreatment with intrathymic injection of donor-specific alloantigen could induce tolerance to sciatic nerve allografts in rats transplantation,in certain range,the level of immunotolerance is relevant to the dose of injected MHC antigen.

关 键 词：胸腺内注射 免疫耐受 神经移植 

分 类 号：R392.4[医药卫生—免疫学]