旋毛虫TsP53重组蛋白对小鼠的免疫保护性  

Immune protection of TsP53 recombinant protein on mice against Trichinella spiralis in mice

作　　者：王鸿盛[1,2] 李文卉[1] 盖文燕[1] 姚菊霞[1] 曲自刚[1] 王艳华[1] 张德林[1] 薛慧文[2] 付宝权[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部兽医公共卫生重点开放实验室甘肃省动物寄生虫病重点实验室,甘肃兰州730046 [2]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《中国兽医科学》2010年第10期1023-1027,共5页Chinese Veterinary Science

基　　金：国家"十一五"科技支撑计划项目(2007BAD40B03);中央级公益性科研院所基本科研业务费专项(0032007012);甘肃省科技重大专项(0702NKDA039)

摘　　要：将旋毛虫TsP53重组蛋白以20μg/只的剂量免疫小鼠3次后攻击感染旋毛虫肌幼虫200条/只。检查小鼠肠道旋毛虫7日龄成虫数、雌虫体外产新生幼虫数和感染35d后的肌幼虫数,并计算减虫率,同时用间接ELISA检测血清中抗TsP53重组蛋白的抗体应答。结果显示,TsP53重组蛋白免疫小鼠旋毛虫7日龄成虫、雌虫产新生幼虫的减虫率分别为14.34%和16.93%,与感染对照组和佐剂对照组相比差异不显著(P>0.05);感染35d后肌幼虫减虫率为38.68%,与感染对照组和佐剂对照组相比差异显著(P<0.05)。TsP53重组蛋白免疫小鼠在首次免疫后第7天出现抗体应答,可检测到抗TsP53重组蛋白的抗体,第3次免疫后第7天达到峰值,攻击感染后略有下降,但直到试验结束时仍然维持在较高水平,TsP53重组蛋白可诱导小鼠产生部分抗旋毛虫的免疫保护性。Forty mice in immunization group were immunized with 20μg TsP53recombinant protein 3 times and 30mice in adjuvant group and 30mice in infection control group were challenged with 200infective larvae of Trichinella spiralis per mouse on day 7post-3rd-immunization.The reduction rates of 7-day-old adult worms,production of newborn larvae of female adult in vitro and muscle larvae on day 35post-in- fection were counted and the anti-TsP53IgG level in the sera of immunized animals was detected by indi- rect ELISA.The reduction rate of 7-day-old adult worms,newborn larvae and muscle larvae were 14.34%, 16.93%and 38.68%,respectively.TsP53-specific antibody response in the immunized mice was detectable on day 7post-1st-immunization and reached the peak on day 7post-3rd-immunization.The anti-TsP53 IgG level in the immunized mice was significantly higher than that in the control groups.The result showed that the TsP53 recombinant antigen could induce partial immune protection in mice against T.spiralis.

关 键 词：旋毛虫 TsP53 重组蛋白 免疫保护性 

分 类 号：S852.731[农业科学—基础兽医学]