水菖蒲杀虫活性组分菖蒲螺酮的分离与鉴定(英文)  

Isolation and characterization of the insecticidal constituent acorone from Acorus calamus(Araceae)

作　　者：姚英娟[1,2] 蔡万伦[1] 杨长举[1] 张宏宇[1] 华红霞[1] 

机构地区：[1]华中农业大学城市有害生物防治研究所,武汉430070 [2]江西省农业科学院植物保护研究所,南昌330200

出　　处：《昆虫学报》2010年第9期985-992,共8页Acta Entomologica Sinica

基　　金：国家农业公益性行业科研专项(200803003)

摘　　要：储粮害虫的危害造成储粮严重损失,化学农药的种种弊端使开发新型环境友好型药剂非常迫切,植物由于其自身的特点,成为开发新型药剂的重要来源。我们的前期研究表明,水菖蒲Acorus calamus提取物对多种储粮害虫具有明显的触杀活性,并且分离得到了其主要活性组分β-细辛醚。本研究采用硅胶柱层析法,并以对玉米象Sitophilus zeamais的触杀活性进行追踪,对水菖蒲提取物进行分离,得到另一活性组分。经气相色谱-质谱联用、红外吸收光谱和核磁共振波谱方法鉴定,该活性组分为单体化合物菖蒲螺酮(1-异丙基-4,8-二甲基螺[4.5〗癸-2,7-二酮)。将菖蒲螺酮以314.54,251.63,188.72,125.82和62.91μg/cm2的浓度处理玉米象96h后,玉米象的死亡率分别为85.6%,72.2%,53.3%,38.9%和15.6%。处理96h后,菖蒲螺酮对玉米象成虫的LD50为158.00μg/cm2。这些结果说明菖蒲螺酮对玉米象具有明显的生物活性,是水菖蒲提取物中除β-细辛醚以外的又一活性组分。The stored-grain insect pests can cause serious quantitative and qualitative losses of stored-grain.Because of the negative effects of the synthetic insecticides,there is an urgent need to develop new types of environmental-friendly insecticides,of which plant has become a new potential resource.Our previous study showed that the extract of the traditional medicinal plant Acorus calamus had toxicity to stored-grain insects and（Z）-asarone was found to be one of the active constituents from its extract.In this study,the extract of A.calamus was separated and another active constituent of A.calamus extract was obtained by choromatographing and bioassay-guiding its contact toxicity to Sitophilus zeamais.Using spectroscopic analysis,including GC-MS,IR,1H NMR,13C NMR,and single crystal X-ray diffraction,the active constituent was identified as a single sesquiterpenoid compound acorone（1-isopropyl-4,8-dimethyl-spiro ;4.5] decane-2,7-dione）.When S.zeamais was treated for 96 h with acorone at the concentration of 314.54,251.63,188.72,125.82 and 62.91 μg/cm2,its mortalities were 85.6%,72.2%,53.3%,38.9% and 15.6%,respectively.LD50 at 96 h after treatment was 158.00 μg/cm2.We so concluded that acorone has significant contact toxicity to S.zeamais and is another active constituent besides（Z）-asarone.

关 键 词：水菖蒲 菖蒲螺酮 活性组分 触杀活性 玉米象 

分 类 号：Q965.9[生物学—昆虫学]