新生期大鼠接种极低密度脂蛋白对部分免疫学及生物化学指标的影响  

Effects of Vaccination with Very Low Density Lipoprotein on Some Immunological and Biochemical Indicators in Neonatal Rats

作　　者：尹翠萍[1] 侯桂华[1] 冯月秋[1] 孔峰[1] 梁婷[1] 王束玫[1] 

机构地区：[1]山东大学公共卫生学院流行病与统计学教研室,山东省济南市250012

出　　处：《中国动脉硬化杂志》2010年第8期630-632,共3页Chinese Journal of Arteriosclerosis

摘　　要：目的探讨新生期大鼠接种极低密度脂蛋白是否能产生免疫耐受,以及接种后对血脂和内皮素水平的影响。方法选取体重在150-180 g雄性Wistar大鼠40只,制作高脂模型后取血,梯度密度离心提取极低密度脂蛋白并测定其蛋白含量。将出生4 h内的Wistar雄性乳鼠按窝别随机分为两组,极低密度脂蛋白组腹腔注射极低密度脂蛋白0.3 mg,对照组注射等体积生理盐水,普通饲料喂养3周。从两组大鼠中各随机抽取10只腹腔注射极低密度脂蛋白,喂养15天后取血测血清抗极低密度脂蛋白水平;剩余大鼠高脂饲料喂养60天,取血测血脂、内皮素及T细胞增殖水平。结果与对照组相比,极低密度脂蛋白组抗体水平降低（P〈0.05）,T细胞增殖水平有所下降（P〈0.05）,而血脂及内皮素水平变化不明显。结论新生期大鼠接种极低密度脂蛋白可以诱导免疫耐受。Aim To study whether very low density lipoprotein（VLDL） vaccination in neonatal rats could induce immue tolerance against VLDL and explore the neonatal vaccination＇s effects on blood lipids and endothelin. Methods 40 Wistar male rats（weight 150～180 g） were selected,hyperlipidemic rats were built,VLDL were distilled by density-gradient centrifuge and the protein content was measured.The neonatal Wistar male rats were selected within 4 hours and divided into two groups randomly,while VLDL group was intraperitoneally injected 0.3 mg VLDL and control group saline.After 3 weeks,part of rats with VLDL were challenged,the serum anti-VLDL was measured.Then the rest of rats were given high fat diet for 60 days,the capacity of T cell proliferation,the level of serum lipids and the level of serum endothelin were measured respectively. Results Compared with control group,serum anti-VLDL and the capacity of T cell proliferation in VLDL group were significantly reduced（P〈0.05）.There were no significant difference on serum lipids and endothelin between the two groups. Conclusion Neonatal VLDL vaccination could induce specific immune tolerance.

关 键 词：动脉粥样硬化 极低密度脂蛋白 免疫耐受 血脂 

分 类 号：R392[医药卫生—免疫学]