高温胁迫下外源NO对高灌蓝莓PSⅡ光化学活性和抗氧化系统的影响  

Effects of exogenous nitric oxide on highbush blueberry PSⅡ photochemical activity and antioxidant system under high temperature stress

作　　者：魏海蓉[1] 孟艳玲 孙阳[3] 刘庆忠[1] 

机构地区：[1]山东省果树研究所,山东泰安271000 [2]威海市农业科学院,山东威海264200 [3]辽宁省经济林研究所,辽宁大连116031

出　　处：《应用生态学报》2010年第10期2529-2535,共7页Chinese Journal of Applied Ecology

基　　金：公益性行业(农业)科研专项(nyhyzx07-028);山东省农业科学院创新基金项目(2007YCX010)资助

摘　　要：以高灌蓝莓品种‘都克’试管苗移栽到田间6个月的植株为试材,研究了高温胁迫条件下,外源一氧化氮(NO)对高灌蓝莓幼苗生长、PSⅡ光化学活性和抗氧化系统的影响.结果表明:0.2、0.5和1.0 mmol.L-1外源NO供体硝普钠(SNP)处理显著减缓了高温胁迫对高灌蓝莓光合系统Ⅱ(PSⅡ)的抑制,高灌蓝莓PSⅡ最大光化学效率(Fv/Fm)、实际光化学效率(ΦPSⅡ)、光化学猝灭系数(qP)和非光化学猝灭系数(NPQ)下降缓慢,使光合机构免受高温胁迫的伤害;叶片质膜透性和丙二醛含量降低,而超氧化物歧化酶和过氧化氢酶活性升高,从而促进了脯氨酸的积累.适当浓度的SNP能显著缓解高温胁迫对高灌蓝莓植株的伤害,其中0.5 mmol.L-1 SNP的缓解效果最好.Taking the test tube ‘Duke’highbush blueberry（Vaccinium corymbosum） seedlings al photochemical efficiency under light（ΦPSⅡ）,photochemical quench（qP）,and nonphotochemi-cal quench（NPQ） caused by high temperature,and prevented the damage of high temperature on photosynthetic apparatus.Comparing with the control,treatments NO decreased the leaf membrane permeability and MDA content,increased the SOD and CAT activities significantly,and promoted proline accumulation.Appropriate concentration SNP could significantly alleviate the damage of high temperature stress on highbush blueberry seedlings,and 0.5 mmol·L^-1 of SNP had the mosthaving been transplanted to the field for 6 months as test materials,this paper studied the effects of exogenous nitric oxide（NO） on their growth,PSⅡ photochemical activity,and antioxidant system under high temperature stress.Applying 0.2,0.5,and 1.0 mmol·L^-1 of exogenous sodium nitro-prusside（SNP） could alleviate the decrease of maximum photochemical efficiency（Fv /Fm）,actu-satisfactory effect.

关 键 词：高温胁迫 一氧化氮 高灌蓝莓 PSⅡ光化学活性 抗氧化系统 

分 类 号：S663.9[农业科学—果树学]