马尾松纯林中球孢白僵菌种群的遗传异质性  

Genetic heterogeneity of Beauveria bassiana population in pure Masson’s pine plantation

作　　者：陈名君[1] 黄勃[1] 刘玉军[1] 李增智[1] 

机构地区：[1]安徽农业大学微生物防治安徽省重点实验室,合肥230036

出　　处：《应用生态学报》2010年第10期2627-2634,共8页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(30972368);安徽省优秀青年科技基金项目(08040106902);安徽省级自然科学研究重点项目(TD200708);安徽农业大学校青年科学基金项目资助

摘　　要：应用ISSR分子标记对安徽省麻姑山马尾松人工纯林的111株球孢白僵菌进行遗传异质性分析,7个引物共得到58个位点,其中多态位点比率为93.10%.不同调查时间亚种群的Nei基因多样性(He)为0.2552,Shannon信息指数(I)为0.3825,亚种群间的基因分化系数(Gst)为0.2269,基因流(Nm)为0.8518,其中5月和7月间的遗传距离最小,为0.0408;不同寄主亚种群的He为0.2623、I为0.3884,亚种群间的Gst为0.1964、Nm为1.0223,其中鞘翅目和鳞翅目间亚种群的遗传距离最小,为0.0163.表明麻姑山球孢白僵菌存在较高的遗传异质性,亚种群间的遗传变异较小,亚种群内表现出相对较大的遗传变异,未见ISSR图谱相同或高度相似的菌株.By the method of ISSR,the genetic heterogeneity of 111 Beauveria bassiana isolates in a pure Masson’s pine plantation in Magu Mountains of Anhui Province was analyzed.A total of 58loci were obtained with 7 ISSR primers,54 of which（93.10%） were polymorphic.For the isolatessampled at different time,their Nei’s gene diversity（He） was 0.2552,Shannon’s diversity index（I） was 0.3825,coefficient of gene differentiation（Gst） was 0.2269,and gene flow Nm was 0.8518.The genetic distance between the isolates sampled in May and July was the shortest B.bassiana population in the test plantation was rich,and the genetic variation between B.bassi-ana subpopulations was relatively low but that within the sub-populations was high,and none of the（0.0408）.For the isolates grouped based on host orders,their He was 0.2623,I was 0.3884,Gstwas 0.1964,and Nm was 1.0223.The genetic distance between the isolates from Coleopteran andHymenoteran was the shortest（0.0163）.All the results suggested that the genetic heterogeneity of isolates were identical or highly similar with each other.

关 键 词：虫生真菌 分子标记 基因流 遗传分化 遗传多样性 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]