柱后衍生化高效液相色谱法测定妥布霉素滴眼液的含量  

Determination of tobramycin sulfate drops by HPLC with post-column derivatization

作　　者：何丹[1] 杨林 

机构地区：[1]重庆医科大学药学院重庆市药物工程研究中心重庆市生物化学与分子药理学重点实验室,重庆400016 [2]重庆市药品检验所,重庆400015

出　　处：《中国抗生素杂志》2010年第10期771-773,共3页Chinese Journal of Antibiotics

摘　　要：目的 建立高效液相色谱-柱后衍生法测定硫酸妥布霉素含量的方法。方法色谱柱为watersSunFireC18（4．6mm×150mm，5μm）；流动相为含戊烷磺酸钠和冰醋酸的硫酸钠溶液；柱温30℃，流速1．2mL／min。柱后衍生化试液为邻苯二甲醛溶液，流速0．5mL／min，衍生化反应温度60℃，荧光激发波长360nm，发射波长440nm。结果硫酸妥布霉素在4．15-62．28mg／L浓度范围内线性关系良好（r=0．9999），平均回收率0=9）为99．5％。结论该方法简便快速，结果准确可靠，重复性好，可用于硫酸妥布霉素的定量分析。Objective To establish a method using HPLC with post-column derivatization for detecting tobramycin sulfate drops. Methods A Waters SunFire C18 column（4. 6mm×150mm, 5μm） was used, and the mobile phase was sodium sulfate solution （contain pentanesulfonate sodium salt and glacial acetic acid）, column temperature was 30℃, at a flow rate of 1. 2mL/min. OPA solution was used for post-column derivatization online, at a flow rate of 0. 5mL/min. The temperature of post-column reaction was set at 60℃, detected by fluorescence detector （λex 360nm, ）λem 440nm）. Results The calibration curve was linear in the range of 4. 15-62. 28mg/L, r-=0. 9999. The average recovery（n=9）was 99. 5%. Conclusion The method presented in this study showed good accuracy, more precision, simplicity and reproducibility as a recommendable option for quality control oftobramvcin sulfate drops.

关 键 词：妥布霉素 高效液相色谱法 柱后衍生 荧光检测 

分 类 号：R978.1[医药卫生—药品]