谷氨酰胺对内毒素血症幼鼠肠屏障的保护作用  

Effect of oral glutamine on intestinal barrier function in yaung rats with endotoxemia

作　　者：常晓[1] 王琳琳[2] 连淑君[2] 唐清[2] 陈萍[2] 王华[2] 

机构地区：[1]新乡医学院第一附属医院新生儿科,河南卫辉453100 [2]广西医科大学第一附属医院儿科,广西南宁530021

出　　处：《中国当代儿科杂志》2010年第10期809-811,共3页Chinese Journal of Contemporary Pediatrics

基　　金：广西自然科学基金(桂科自0640086)

摘　　要：目的观察内毒素血症幼鼠经谷氨酰胺(Gln)干预后血浆D-乳酸、血浆及小肠二胺氧化酶(DAO)浓度变化,以探讨Gln对肠屏障功能的干预作用。方法 18日龄Wistar大鼠80只,随机分为内毒素血症组、Gln干预组,每组40只,各组根据注射内毒素后取标本时间分为1.5h、6h、24h、72h和7d等5个亚组(n=8)。Gln干预组在注射内毒素后立即予以Gln口服(2g/kg),随后每天1次。各组分别取血浆及小肠匀浆,测定血浆DAO活性及D-乳酸、小肠匀浆DAO值。结果①Gln干预组6h、72h血浆DAO活性明显低于内毒素组(P<0.05)。②Gln干预组6h、24h、72h及7d的肠组织DAO活性较内毒素组均明显增高(P<0.05,P<0.01)。③Gln干预组6h、24h、72h、7d血浆D-乳酸含量明显低于内毒素组(P<0.01)。结论 Gln能降低内毒素血症大鼠肠道黏膜通透性,从而具有保护肠屏障功能。Objective To study the effect of glutamine on intestinal barrier function by examining the changes of plasma D-lactic levels and diamine oxidase （ DAO） levels in plasma and intestinal tissue after glutamine intervention in young rats with endotoxemia. Methods Eighty 18-day-old rats were randomly divided into endotoxemia and glutamine intervention groups （ n = 40 each） . Endotoxemia was induced by lipopolysaccharide （ LPS） injection. Plasma and small intestine homogenate were collected 1. 5,6,24 and 72 hrs and 7 days after LPS injection. The glutamine intervention group was immediately administered with oral glutamine （ 2 g /kg ） after LPS injection. Afterwards, glutamine was administered once daily. Plasma D-lactic and DAO levels and intestinal DAO levels were measured. Results Plasma DAO activity in the glutamine intervention group was significantly lower than that in the endotoxemia group 6 and 72 hrs after LPS injection （ P 0. 05） . In contrast,the intestinal DAO activity in the glutamine intervention group was significantly higher than that in the endotoxemia group 6,24 and 72 hrs and 7 days after LPS injection （ P 0. 05 or 0. 01） . Plasma D-lactic levels in the glutamine intervention group were significantly lower than those in the endotoxemia group 6,24 and 72 hrs and 7 days after LPS injection （ P 0. 01） . Conclusions Glutamine may reduce the permeability of intestinal mucosa,and thus provides protective effects on intestinal barrier function in rats with endotoxemia.

关 键 词：谷氨酰胺 D-乳酸 二胺氧化酶 内毒素血症 幼鼠 

分 类 号：R720.597[医药卫生—急诊医学]