复合微生物固体发酵玉米秸秆中试工艺研究  

Study on pilot-scale techniques of corn straw in solid state mixed fermentation by multi-microorganism

作　　者：秦涛[1] 马齐[1] 张强[1] 李忠玲[2] 

机构地区：[1]陕西省科学院酶工程研究所,陕西西安710600 [2]陕西省酶工程技术中心,陕西西安710600

出　　处：《黑龙江畜牧兽医》2010年第10期8-9,14,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家星火计划项目(2007EA850003)

摘　　要：为了确定最佳发酵工艺条件,试验采用黄孢原毛平革菌、绿色木霉进行了混合固体发酵玉米秸秆工艺条件的中试研究。结果表明:固体发酵培养基的组成为玉米秸秆与麸皮为4∶1,加入5%的尿素、0.5%的KH2PO4、0.1%的MgSO4.7H2O;加水量为1∶1.3;发酵温度为32℃;接种比例为2∶1;接种时间为接入黄孢原毛平革菌发酵48 h后接入绿色木霉;发酵周期为120 h。混合固体发酵后含有纤维素酶420 U/g,木质素酶85 U/g,粗纤维的利用率为69%。To study pilot-scale technology and parameters by mixed ferment of corn straw of Phanerochaete chrysosporium and Trichoderma viride based on the experiment to the optimization of technology,the experiment adopted the ratio of solid culture medium was 4∶1（corn straw/wheat bran）,with 5% urea,0.5% potassium dihydrogen phosphate and 0.1% Magnesium sulfate heptahydrate.The ratio of the nutrients to water was 1∶1.3,the ferment temperature was 32 ℃,the ratio of inoculation was 2∶1（Trichoderma viride /Phanerochaete chrysosporium）.Trichoderma viride was inoculated after 48 hours of Phanerochaete chrysosporium inoculation,and the time of fermentation were five days.The results of mixed ferment technology showed that cellulase（FPA） activity reached 420 U/g,ligninase activity reached 85 U/g,and the degradation rate of crude fiber reached 69%.

关 键 词：绿色木霉 黄孢原毛平革菌 混合发酵 木质素酶 纤维素酶 

分 类 号：S816.6[农业科学—饲料科学]