寒冷诱导产热时大鼠棕色脂肪能量代谢相关蛋白谱的变化(英文)  

The Alterations of Energy Metabolism-related Protein Patterns in Brown Adipose Tissue of Rats During Cold-induced Thermogenesis

作　　者：杨悦[1,2] 龚未[1,2] 王伟伟[1,2] 俞晓敏[1] 胡松年[1] 于军[1] 

机构地区：[1]中国科学院北京基因组研究所,基因组科学及信息重点实验室,北京100029 [2]中国科学院研究生院,北京100049

出　　处：《生物化学与生物物理进展》2010年第10期1108-1119,共12页Progress In Biochemistry and Biophysics

摘　　要：棕色脂肪组织(BAT)的生理作用与白色脂肪显著不同,它以产热的形式释放能量而不是将能量以 ATP的形式储存.线粒体是在能量代谢和维持细胞稳态中具有重要功能的细胞器.为了更好地了解棕色脂肪中的能量代谢过程,运用双向电泳及质谱相结合的技术,分离了大鼠白色和棕色脂肪线粒体,对其差异蛋白质谱进行了系统分析和鉴定.参与脂肪和氨基酸代谢、三羧酸循环及线粒体呼吸链的蛋白质在棕色脂肪线粒体中的表达明显高于白色脂肪线粒体,在寒冷诱导下这些蛋白质的表达进一步上调.此外,参与辅酶 Q 合成的一系列 COQ 基因在棕色脂肪中经寒冷适应后表达明显上调.该研究表明,辅酶 Q合成的增高在非颤栗性产热中具有重要作用,为进一步了解棕色脂肪特异性的能量代谢提供了新的思路.The physiological role of brown adipose tissue （BAT） is quite different from that of white adipose tissue （WAT） by dissipating energy as heat instead of generating ATP. Mitochondrion is an essential organelle that plays a crucial role in energy production and cellular homeostasis. To gain a better understanding of energy metabolism in BAT, mitochondria were isolated and a systematic analysis of protein patterns was performed in WAT and BAT of rat with two-dimensional gel electrophoresis. Differentially expressed proteins were identified using mass spectrometry and it was found that the proteins involved in lipid and amino acid metabolisms, TCA cycle, and respiratory chain are more abundant in BAT than in WAT, which are further up-regulated in response to cold exposure in BAT. Furthermore, the study reveals, for the first time, a subset of COQ genes required for Coenzyme Q biosynthesis are significantly up-regulated after cold exposure in BAT. These findings suggest that Coenzyme Q increase is implicated in the non-shivering thermogenesis and contribute new data for understanding the unique process of energy metabolism in BAT.

关 键 词：棕色脂肪组织 寒冷适应 产热 辅酶Q 

分 类 号：Q256[生物学—细胞生物学] Q413