条锈菌诱导的小麦C3HC4型锌指蛋白基因的克隆及其特征分析  

Cloning and Characterization of a C3HC4-type Zinc Finger Gene in Wheat Induced by Stripe Rust Pathogen

作　　者：张毅 张岗[2] 徐进 郭军[2] 黄丽丽[2] 康振生[2] 

机构地区：[1]西安市植保植检站,西安710061 [2]西北农林科技大学植物保护学院,陕西省农业分子生物学重点实验室,陕西杨凌712100

出　　处：《西北农业学报》2010年第10期49-54,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家重点基础研究发展计划(973计划)项目(2006CB708208);国家高新技术研究发展计划(863计划)重大研究项目(2006AA10A104);教育部科学技术研究重点项目(107104);教育部长江学者和创新团队发展计划项目(IRT0558);国家自然科学基金项目(30671350);高等学校学科创新引智计划项目(B07049)

摘　　要：应用电子克隆和RT-PCR方法,从小麦叶片中首次分离出1个条锈菌诱导的编码C3HC4型锌指蛋白基因的cDNA序列,暂被命名为TaZFP1。TaZFP1包含一个完整的849 bp的开放阅读框,编码282个氨基酸,具有C3HC4保守结构域;小麦TaZFP1氨基酸序列与水稻OsZFP1相似性达89%,与玉米ZmZFP1相似性达81%。TaZFP1在小麦根、茎、叶组织中表达量基本一致。在小麦与条锈菌的亲和、非亲和互作中,TaZFP1基因均受条锈菌诱导高水平表达,且非亲和组合表达量高于亲和组合,表明TaZFP1可能参与小麦对条锈菌的防御反应。To better understand wheat（Triticum aestivum L.） defense responses to Puccinia striiformis f.sp.tritici,the cDNA library of wheat leaves infected by Puccinia striiformis f.sp.tritici is constructed in our laboratory.On the basis of previous study,a new C3HC4-type zinc finger gene was isolated from this cDNA library through in-silico cloning and RT-PCR approaches.The gene was tentatively designated as TaZFP1,whose open reading frame was 849 bp in length and encoded 282 amino acids containing a conserved C3HC4 domain.The amino acid sequence of TaZFP1 shares 89% identify with OsZFP1 in rice and 81% identify with ZmZFP1 in Zea mays.The expression of TaZFP1 gene was at a similar level in leaves,stems,and roots.The Realtime PCR results revealed that TaZFP1 was up-regulated in both compatible and incompatible interactions.However,the expression in incompatible interaction was higher than that in compatible interaction,suggesting that TaZFP1 may participate in defense response of wheat resistance to stripe rust pathogen.

关 键 词：条锈菌 小麦 C3HC4型锌指蛋白 电子克隆 基因表达 

分 类 号：S512.11[农业科学—作物学]