蛋白激酶C在未成熟心肌预处理中的作用  

The Role of Protein Kinase C in Immature Myocardial Ischemic Preconditioning

作　　者：孙忠东[1] 高尚志[2] 王志维[2] 邢建洲[2] 毛志福[2] 

机构地区：[1]青岛大学医学院附属青岛市立医院心外科,山东青岛266071 [2]武汉大学人民医院胸心外科,武汉430060

出　　处：《中国胸心血管外科临床杂志》2010年第5期395-398,共4页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

摘　　要：目的为进一步加强未成熟心肌保护和临床应用的可行性,探讨蛋白激酶C(PKC)在未成熟心肌预处理保护中的作用。方法建立兔Langendorff灌注模型,将24只幼兔随机分为4组:缺血再灌注组(I/R组)、心脏缺血预处理组(MIP组)、PKC阻滞组(CLT组)和PKC激活剂组(PKC组),观察4组幼兔血流动力学、生化、心肌超微结构等指标。结果 MIP组和PKC组心功能恢复、心肌含水量优于I/R组和CLT组(P<0.01),三磷酸腺苷(ATP)含量、超氧化物歧化酶活性、心肌线粒体Ca2+-ATPase活性、心肌线粒体合成ATP的能力优于I/R组和CLT组(P<0.01),丙二醛含量、血清肌酸激酶和乳酸脱氢酶漏出率、心肌细胞内Ca2+含量、心肌线粒体Ca2+含量低于I/R组和CLT组(P<0.01),心肌超微结构损伤较I/R组和CLT组明显减轻。结论心肌缺血预处理对未成熟心肌具有明显的保护作用,其机制可能是通过PKC的激活起作用。Objective To investigate the mechanism of protein kinase C（PKC） in immature myocardial ischemic preconditioning in order to further its clinical applicability. Methods Langendorff perfusion heart models of 24 rabbits were set up and they were randomly divided into 4 groups： ischemic reperfusion group（I/R group）,myocardial ischemic preconditioning group（MIP group）,chelerythrine group（CLT group） and protein kinase C group（PKC group）.The hemodynamics,biochemistry and myocardial ultrastructure were observed.Results The heart function recovery and myocardial water content in the MIP and the PKC groups were better than those of the I/R and the CLT groups（P〈0.01）.The adenosine triphosphate（ATP） content,superoxide dismutase activity,mitochondrial Ca2＋-ATPase activity and synthesizing ATP activity of mitochondria in the MIP and the PKC groups were significantly higher than those of the I/R and the CLT groups（P〈0.01）.The dehydrogenase and creatine kinase leakage,malondialdehyde content,myocardial cell Ca2＋ content and mitochondrial Ca2＋ content in the MIP and the PKC groups were significantly lower than those of the I/R and the CLT groups（P〈0.01）.The myocardial ultrastructure injuries in the MIP and the PKC groups were less than that of the I/R and the CLT groups.Conclusion Myocardial ischemic preconditioning plays an important role in protecting immature myocardium,which is probably realized by the activation of PKC.

关 键 词：未成熟心肌 心肌保护 蛋白激酶C 

分 类 号：R654.1[医药卫生—外科学]