人巨细胞病毒临床株UL146基因多态性研究  

Polymorphism of Human Cytomegalovirus (HCMV) UL146 Gene

作　　者：常淑婷[1] 毛志芹[1] 易彪[1] 尹晓波[1] 阮强[1] 何蓉[1] 吉耀华[1] 孙峥嵘[1] 马艳萍[1] 齐莹[1] 

机构地区：[1]中国医科大学附属盛京医院儿科,沈阳110004

出　　处：《中国医科大学学报》2010年第9期749-751,共3页Journal of China Medical University

基　　金：辽宁省科技攻关计划课题(2007225005-5);国家自然科学基金资助项目(30700916)

摘　　要：目的研究人巨细胞病毒(HCMV)UL146基因在巨细胞病毒感染临床株中的多态性,寻找具有临床诊断、治疗价值的基因,为HCMV感染的临床诊断和防治提供资料。方法选择经QPCR检测尿HCMV-DNA为阳性的临床株进行UL146基因序列PCR扩增,对扩增阳性标本进行序列测定和分析。结果 28株扩增阳性标本序列之间有很大变异,种系树化分析结果显示可以分为3个型,4个亚型;ELRCXC的趋化因子功能区在各序列中高度保守。结论 HCMV-UL146基因呈现高度多态性,其编码的ELRCXC趋化因子功能区在各序列中高度保守,HCMV-UL146基因多态性与HCMV感染不同临床症状之间的关系尚不明确。Objective To investigate the polymorphism of human cytomegalovirus（HCMV）UL146 gene in clinical strains,and to evaluate its clinical diagnostic and therapeutic value of gene.Methods The UL146 gene of clinical strains was examined by quantitative polymerase chain reaction（Q-PCR）or general polymerase chain reaction（PCR）.Positive samples of PCR amplification were sequenced and analyzed.Results High variability of UL146 gene was found among 28 HCMV strains.According to phylogenetic analysis,all sequences of UL146 in clinical strains could be divided into three types and four subtypes.Chemokine ELRCXC region was highly conserved in all sequences.Conclusion HCMV-UL146 genes showed a high degree of polymorphism,and its encoded chemokine ELRCXC region was highly con-served.The relationship between HCMV-UL146 gene′s polymorphism and different clinical symptoms of HCMV infection was unclear.

关 键 词：人巨细胞病毒 UL146基因 多态性 趋化因子 

分 类 号：R723[医药卫生—儿科]