新疆伊犁地区马脑组织尼帕病毒的感染情况  

Nipah Virus Infection in Horse Brain Tissues in Ili Prefecture, Xinjiang Uygur Autonomous Region, China

作　　者：冯裕星[1,2] 何丰[1,2] 谢亮[1,2] 胡子成[1,2] 孙后超[1,2] 徐红波[1,2] 展群岭[1,2] 徐鸣明[1,2] 张亮[1,2] 胡永波[1,2] 李雷雷[2] 谢鹏[1,2] 

机构地区：[1]重庆医科大学附属第一医院神经内科,重庆400016 [2]重庆医科大学神经科学研究中心,重庆400016

出　　处：《中国生物制品学杂志》2010年第10期1050-1054,共5页Chinese Journal of Biologicals

基　　金：国家重大科学研究计划(973计划)项目(2009CB-918302);"十一五"国家高技术研究发展计划(863计划)项目(2006AA02Z196)

摘　　要：目的采用一步法荧光定量RT-PCR检测新疆伊犁地区天然牧场放养马群脑组织尼帕病毒(NipahVirus,NiV)核蛋白N基因的表达,调查该地区马群中枢神经系统NiV感染的流行状况。方法针对NiV高度保守区N基因设计特异性引物和探针,建立检测马脑组织样本中低浓度NiV RNA的一步法荧光定量RT-PCR方法,对该方法的敏感性及特异性进行验证,并对新疆伊犁地区天然牧场放养且未接种NiV疫苗的183匹马脑组织进行检测。结果一步法荧光定量RT-PCR的最低检出限为1.1×102 copies/μl;与NiV同为亨尼帕病毒属,且高度同源的亨德拉病毒(Hendra Virus,HeV)及同为单股负链的博尔纳病病毒(Borna Disease Virus,BDV)均无交叉反应;183份马脑组织样本未检出阳性样本。结论新疆伊犁地区天然牧场放养马匹中未发现NiV感染,该地区短时间内爆发NiV的可能性较小。Objective To investigate the Nipah virus（NiV）infection in the brain tissue of grazing horses in Ili Perfecture, Xinjiang Uygur Autonomous Region, China by one-step fluorescent quantitative RT-PCR for nucleoprotein（N）gene fragment of NiV. Method Specific primers and probes were designed for detecting N gene fragment which was the highly conservative region of NiV, based on which the one-step fluorescent quantitative RT-PCR method for detection of NiV RNA at a low concentration in samples of central nervous system（CNS）of horses was developed, verified for sensitivity and specificity, and used for the detection of brain tissue samples of 183 grazing horses unimmunized with NiV vaccine. Result The minimum detection limit of the developed one-step fluorescent quantitative RT-PCR method was 1. 1 × 102 copies / μl. No cross reaction was observed with Hendra virus（HeV）belonging to Henipaviruses and highly homologous to NiV or with Borna disease virus（BDV）, another single negative-stranded RNA virus. No positive results were observed in 183 brain tissue samples of horses. Conclusion No NiV infection was found in the grazing horses in Ili Perfecture, indicating low possibility of outbreak of NiV infection in said region in the near future.

关 键 词：尼帕病毒 N基因 一步法荧光定量RT-PCR 新疆伊犁地区 马 

分 类 号：R512.62[医药卫生—内科学]