高选择性脊神经后根切断对SD大鼠膀胱和阴茎勃起功能的影响  

Effects of highly selective dorsal rootlets rhizotomy on bladder function and penis erection in SD rats

作　　者：朱根应[1] 周谋望[1] 黄红拾[1] 陈亚平[1] 王文婷[1] 曾凡硕[1] 

机构地区：[1]北京大学第三医院康复医学科,北京100191

出　　处：《北京大学学报（医学版）》2010年第5期530-534,共5页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金(30672096);北京市科技新星计划(2008A006)资助~~

摘　　要：目的:探讨高选择性切断脊神经后根小束对SD大鼠膀胱和阴茎勃起功能的影响。方法:清洁级成年雄性SD大鼠40只,体重300～350g,其中10只电刺激L6和S1后根,观察膀胱内压(intravesical pressure,IVP)和海绵体内压(intracavernous pressure,ICP)的变化以确定膀胱和海绵体的主要神经传导支;另外30只随机分成A、B两组,每组各15只,A组高选择性切断S1后根传导膀胱逼尿肌的小束,B组高选择性切断S1后根传导阴茎海绵体的小束,观察并记录切断前后IVP和ICP的变化情况。结果:分别电刺激L6和S1后根,IVP变化的差异不显著(P=0.972),ICP变化的差异显著,电刺激L6后根的ICP升高值为(6.88±2.76)cmH2O(1cmH2O=0.098kPa),电刺激S1后根的ICP升高值为(13.05±8.41)cmH2O(P<0.01)。分别电刺激S1左右两侧引起IVP和ICP的变化差异无统计学意义(P值分别为0.623和0.828)。A组S1后根小束切断前后电刺激下IVP的变化差异有统计学意义,切断前的IVP升高值为(14.37±4.89)cmH2O,切断后的IVP升高值为(3.25±1.29)cmH2O(P<0.001),而ICP的变化差异无统计学意义(P=0.153);B组S1后根小束切断前后电刺激下ICP的变化差异有统计学意义,切断前的ICP升高值为(11.97±4.41)cmH2O,切断后的ICP升高值为(2.68±1.01)cmH2O(P<0.001),而IVP的变化差异无统计学意义(P=0.162)。结论:通过显微解剖及电刺激可以区别SD大鼠S1后根主要传导膀胱逼尿肌或阴茎海绵体的不同小束,高选择性切断后可以分别改变膀胱内压和海绵体内压,或可为临床治疗脊髓损伤后痉挛性膀胱同时最大限度保留反射性阴茎勃起功能提供实验基础。Objective：To study the highly selective dorsal rhizotomy influence on bladder and penis erection function of SD rats.Methods：Forty mature male SD rats of clean grades,with weight range of 300-350 g were selected.Ten rats were chosen to do electrophysiology study on dorsal rootlets of L6 and S1 segment.Changes in intravesical pressure （IVP） and intracavernous pressure （ICP） were investigated to define main segments which conducted to bladder and corpus cavernosum.Thirty rats were divided into two groups （A and B） on average at random.In group A,we made highly selective dorsal rhizotomy on the fascicle of conduction bladder detrusor muscle.In group B,we made highly selective dorsal rhizotomy on the fascicle of conduction corpus cavernosum.Changes of IVP and ICP after rhizotomy were investigated and recorded.Results：The changes of IVP during electrostimulation were of no significant variation between L6 and S1 （P=0.972）.With the changes of ICP during electrostimulation,S1 segment was of more significant variation than L6 segment,ICP of S1 was （13.05±8.41）cmH2O（1 cmH2O=0.098 kPa）,while ICP of L6 was （6.88±2.76）cmH2O （P〈0.01）.There was no reasonable variation in IVP and ICP on the left and right dorsal rootlets of S1 segment （P was 0.623 and 0.828 respectively）.In group A,there was significant variation in IVP,IVP of before rhizotomy was （14.37±4.89）cmH2O,while after rhizotomy was （3.25±1.29）cmH2O （P〈0.001） while no obvious variation in ICP （P=0.153） after highly selective rhizotomy on S1 dorsal rootlets.In group B,there was significant variation in ICP,ICP of before rhizotomy was （11.97±4.41）cmH2O,while after rhizotomy was （2.68±1.01）cmH2O （P〈0.001）,but no obvious variation in IVP （P=0.162） after highly selective rhizotomy on S1 dorsal rootlets.Conclusion：SD rats＇ different rootlets of S1 dorsal rootlets can be distinguished by microanatomy and electrostimulation.The IVP and ICP had distinct changes after highly selective dorsal

关 键 词：脊神经根切断术 膀胱 阴茎勃起 大鼠 Sprague-Dawley 

分 类 号：R651.21[医药卫生—外科学]