uPA和PAI-1在乳腺癌血浆中的水平测定及其生物学行为的相关性研究  

Relationship between Plasm Urokinase-type Plasminogen Activator (uPA) and Plasminogen Activator Inhibitor (PAI-1) Level and Biological Behaviors in the Breast Cancer Patients

作　　者：汪青[1] 鞠放[1] 王宁[1] 王雅杰[1] 

机构地区：[1]第二军医大学附属长海医院肿瘤科,上海200433

出　　处：《医学研究杂志》2010年第10期44-49,共6页Journal of Medical Research

基　　金：上海市科学技术委员会科研计划资助项目(06DZ19505);上海市卫生局科研计划项目(2009113)

摘　　要：目的研究血浆中uPA和PAI-1的浓度水平和乳腺癌患者生物学行为相关性,并探讨浓度水平与乳腺癌常见免疫组化指标的相关性。方法利用免疫组化ELISA法检测88例初治乳腺癌患者血浆中uPA和PAI-1的浓度水平,并以39例健康体检者血浆为对照,结合患者的临床病理学资料分析。结果乳腺癌组血浆中uPA和PAI-1水平明显高于健康对照组(P<0.001);乳腺癌血浆中uPA与PAI-1浓度呈正相关(P<0.001);血浆uPA的浓度与乳腺癌的瘤体大小、组织学分级、临床分期、腋窝淋巴结转移分别呈正相关(P<0.05),而与患者年龄、病理类型无关;血浆PAI-1的浓度与乳腺癌的瘤体大小、临床分期、腋窝淋巴结转移分别呈正相关(P<0.05),而与患者年龄、组织学分型、病理类型无关;ER表达阳性乳腺癌患者,血浆uPA及PAI-1浓度值高于ER表达阴性患者(P<0.01);Her-2阳性的乳腺癌患者,血浆中uPA及PAI-1浓度值明显高于Her-2阴性患者(P<0.01)。结论血浆中uPA及PAI-1高表达与乳腺癌的侵袭、转移密切相关;与ER的状态、Her-2表达之间密切相关;对uPA与PAI-1联合检测可能有助于判断乳腺癌的恶性进度及预测其生物学行为。Objective This study is to explore the correlation of the expression levels of uPA and PAl - 1 in plasma and the clinicpathological features of breast cancer. Methods Immunohistoehemistry EI, ISA was used to detected uPA and PAl - 1 in plasma of 88 experimental patients and 39 control populations. Results Plasma eoncentration levels of uPA and PAl - 1 were significantly higher than those in the normal control group（ P 〈 0. 001 ）. uPA in Breast cancer patients had positive correlation with PAt - 1 in the plasma concentration （ P 〈 0.01 ） . The concentration of uPA in plasma had positive correlation with the tumor size of the breast cancer, histological grade, clinical stage, axillary lymph node metastasis （P 〈0.05 ） ,had negative correlation with the patient＇s age, pathological subtype. The concentration of PAl - 1 in plasma had positive correlating with breast cancer tumor size, clinical stage, axillary lymph node metastasis （ P 〈 0.05） ,and had negative correlation with age, histological type, pathological subtype. Concentrations of plasma uPA and PAl - 1 expression in the breast cancer patients with ER - positive expression were higher than the ER - negative patients （ P 〈 0.01 ）. The plasma levels of uPA and PAl - 1 were significantly higher in the breast cancer patients with Her - 2 positive expressiol than Her - 2 negative patients （ P 〈 0.01 ）. Conclusion High uPA and PAl - 1 expression in Breast cancer patients and tumor invasion and metastasis were closely related, and the same to the ER status and Her - 2 expression. Combined detection of malignant breast cancer may help to determine the progress and predict their biological behavior.

关 键 词：乳腺肿瘤 尿激酶型纤溶酶原激活物 人纤溶酶原激活剂抑制物-1 侵袭 转移 

分 类 号：R543.02[医药卫生—心血管疾病]