AB-8大孔吸附树脂纯化柴胡总皂苷工艺条件研究  

Separation and Purification of the Total Glucosides in Bupleurum Scorzonerifolium Willd. with AB-8 Macroporous Adsorption Resin

作　　者：梁卫青[1] 浦锦宝[1] 郑军献[1] 胡轶娟[1] 魏克民[1] 

机构地区：[1]浙江省中医药研究院,杭州310007

出　　处：《医学研究杂志》2010年第10期49-51,共3页Journal of Medical Research

基　　金：浙江省科技厅科研院所研究开发项目(2006F13025)

摘　　要：目的考察AB-8大孔吸附树脂对柴胡总皂苷的分离纯化方法。方法根据柴胡总皂苷的结构特点,考察AB-8树脂的吸附性能,对AB-8吸附树脂纯化柴胡总皂苷的工艺条件进行了筛选,并采用紫外-可见分光光度法对柴胡总皂苷含量进行定量分析。结果 AB-8大孔吸附树脂对柴胡总皂苷具有较好的吸附选择性。吸附纯化的最佳工艺条件为吸附流速1BV/h,以70%乙醇作为洗脱剂,洗脱流速为2BV/h。采用上述工艺条件,产品纯度大于60%。结论 AB-8树脂用于柴胡总皂苷的分离纯化简单有效,易于工业化推广。Objective To study the method for separating and purifying the total glucosides in Bupleurum scorzonerifolium Willd. with AB -8 macroporous adsorption resin. Methods According to the structural characteristic of total glucosides, the resinAB - 8 was systematically studied for its adsorption capability. The technological conditions of the adsorption resin AB - 8 for separation was selected. UV - VIS was used to measure the content of total Glucosides. Results The macroporous adsorption resin AB - 8 can be used to produce the total glucosides with high quality. The optimal technological conditions were： the flow velocity of adsorption, 1BV/h; the eluting reagent, 70% ethanol; eluting velocity, 2BV/h. The purity of the total glueosides was over 60% with the technological conditions respectively. Conclusion It is a simple and efficient method to separate the total glucosides from Bupleurum scorzonerifolium Willd. by usingAB - 8 resin, and can be applied to manufacture.

关 键 词：柴胡 总皂苷 大孔吸附树脂 

分 类 号：R284.2[医药卫生—中药学]