1,25(OH)_2维生素D_3和塞莱昔布协同对5-FU化疗的增敏作用  

Effect of 1,25 (OH)2VitD_3 Combined with Celecoxib on Sensitivity Increasing by 5-FU Chemotherapy.

作　　者：孙根林[1] 鲍扬漪[1] 

机构地区：[1]安徽省合肥市第一人民医院肿瘤科,230061

出　　处：《医学研究杂志》2010年第10期74-77,共4页Journal of Medical Research

基　　金：合肥市科技局重点科研项目(合科2007第15号)

摘　　要：目的研究塞来昔布(特异性环氧合酶-2抑制剂)联合1,25(OH)2维生素D3增强5-FU对结肠癌化疗增敏作用及其机制。方法 3种药物用单药、两药和3药联合干预结肠癌细胞株HT2948h,用ELSA法观察干预后结肠癌细胞抑制率和基质金属蛋白酶-7(MMP-7)表达变化及联用5-FU后溶解型Fas受体(s-FasL)表达的变化。结果单药、两药和3药联合干预结肠癌细胞株HT29后抑制率增加,MMP-7表达减少及s-Fas表达减少(P<0.05或P<0.01)。结论塞莱昔布和1,25(OH)2维生素D3协同对5-FU的增敏作用,其机制可能通过抑制MMP-7表达,降低其对细胞表面Fas的酶解作用,促进结肠癌细胞凋亡。Objective To study the molecular mechanism of enhancing the sensitivity of 5 - FU chemotherapy by celecoxib （a specific COX-2 inhibitors） combined with 1,25 （OH） 2VitD3 in the colon cancer cells. Methods The colon cancer cell lines HT29 were intervented with one drug, two drugs and three drugs for 48h. Determination of MMP - 7 and s - FasL titer was done through cell culture supernatant by means of ELISA. After the determination of OD values was done by means of MTT, inhibition rates were calculated. Results After HT29 cells were treated by one drug, two drugs and three drugs, the inhibition rate increased, and the concentration of MMP -7 and s - Fas decreasing was observed （P 〈 0.05 or P 〈 0.01 ）. Conclusion The molecular mechanism of enhancing the sensitivity of 5 - FU chemotherapy by celecoxib and 1,25 （OH）2 VitD3 in the colon cancer cells may be inhibiting the expression of MMP -7, reducing the shedding of Fas in cell surface by MMP -7 ,and promoting apoptosis of the colon cancer cells in 5 - FU chemotherapy.

关 键 词：1 25(OH)2维生素D3 5-FU 敏感性 

分 类 号：R473.73[医药卫生—护理学]