胰岛素对糖尿病大鼠肾小球蛋白激酶C和核因子κB表达的影响  

Effect of Insulin on the Expression of Protein Kinase C and Nuclear Factor Kappa B in Diabetic Rat Renal Glomerulus

作　　者：肖醉萱[1] 邓德明[1] 孙爱萍[2] 

机构地区：[1]长江大学医学院,荆州434000 [2]长江大学附属第一医院,荆州434000

出　　处：《医学研究杂志》2010年第10期85-87,136,共4页Journal of Medical Research

摘　　要：目的通过观察糖尿病大鼠肾小球病理改变及蛋白激酶C(PKC)、核因子κB(NF-κB)表达的变化,探讨胰岛素的抗炎机制与对PKC、NF-κB信号转导通路抑制的相关性。方法将实验动物随机分为正常对照组(N组)、糖尿病组(DM组)、胰岛素治疗组(INS组)、二甲双胍治疗组(MET组),第4周、8周观察其肾小球病理改变,采用免疫组化方法测定PKC、NF-κB活性,及其在糖尿病大鼠肾小球的表达变化。结果 INS组大鼠血糖水平低于DM组高于MET组,但其肾小球PKC、NF-κB的表达明显低于DM组、MET组。结论胰岛素对PKC、NF-κB表达的抑制应更多为其抗炎作用所致,而并非完全依靠其降糖作用。Objective To study the correlation between the anti - inflammatory mechanisms of insulin effect and the inhibition to protein kinase C （PKC） , nuclear factor kappa B （ NF -κB） signal transduction pathway by observing the pathological changes and PKC, NF -κB expression in glomeruli of diabetic rats. Methods The experimental animals were randomly divided into normal control group （ N group）, diabetic group （DM group）, insulin- treated group （INS group）, metformin- treated group （MET group）. The pathological changes of glomerular on the first 4 weeks, 8 weeks were observed. PKC and NF -κB activity was measured by immunohistochemistry,and the expression changes in diabetic rat glomerular was studied. Results Blood glucose level of INS rats was lower than that of DM group and higher than that of ME group,but its glomerular PKC and NF -κB expression was significantly lower than that of the DM group and MET group. Conclusion The inhibition effect of insulin on PKC and NF -κB expression should be more due to its anti - inflammatory effect, and not rely on its hypoglycemic effect entirely.

关 键 词：胰岛素 PKC NF-ΚB 

分 类 号：R587.1[医药卫生—内分泌]