苏太猪FUT1基因M307位点多态性及其对部分免疫指标的遗传效应分析  

Polymorphism and Genetic Effect of FUT1 Gene M307 on Some Immune Parameters in Sutai Pigs

作　　者：包文斌[1,2] 叶兰[1] 潘章源[1] 朱璟[1] 黄雪根 华金第 吴圣龙[1,2] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]江苏省扬州规模猪场高效健康养殖公共技术服务中心,扬州225009 [3]苏州市苏太猪育种中心,苏州215000

出　　处：《畜牧兽医学报》2010年第10期1219-1224,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：转基因生物新品种培育科技重大专项(2009ZX08006-004B);江苏省科技支撑计划(农业)(BE2008364);江苏省高校自然科学基础研究项目(08KJB230004)

摘　　要：本研究旨在分析苏太猪抗F18大肠杆菌病育种基础群FUT1基因M307位点对部分免疫指标的遗传效应,为下一步苏太猪抗病育种工作提供一定的理论依据。采用PCR-RFLP方法对苏太猪抗F18大肠杆菌病育种基础群FUT1基因M307多态性进行分析,并探讨其与部分免疫指标的关系。结果表明,基础群中576个个体FUT1基因M307位点经Hin6Ⅰ酶切后,产生AA、AG和GG3种基因型,基因型频率分别为0.235、0.609和0.156,抗性基因A为优势等位基因,频率为0.540;群体显著偏离了Hardy-Weinberg平衡状态(P<0.01)。AA基因型个体的血红蛋白(HGB)和白细胞计数(WTBC)显著高于AG和GG型个体(P<0.05),AG和GG型个体间差异不显著(P>0.05);AA基因型个体的异嗜性粒细胞与淋巴细胞比率(H/L值)显著低于AG和GG型个体(P<0.05),AG和GG型个体间差异不显著(P>0.05);AA基因型个体与AG和GG型个体间SRBC抗体滴度差异不显著(P>0.05)。本试验结果初步说明苏太猪F18抗性型群体具有抗逆性强的优良特性,AA基因型不仅对仔猪断奶后水肿病和腹泻病具有抗性,而且具有较高的一般抗病力。This study was conducted to analyze genetic effect of FUT1 gene M307 on some immune parameters in foundation population of Sutai ETEC F18 resistant breeding pigs and aimed to provide a theoretical foundation for further anti-disease breeding of Sutai pigs.The genetic variations at M307 of FUT1 gene in foundation population of Sutai ETEC F18 resistant breeding pigs（total 576 individuals） were investigated by PCR-RFLP method.The relationship between FUT1 gene and some immune parameters were analyzed in this study.The results showed that M307 of FUT1 gene digested by Hin6Ⅰcould be divided into three kinds of genotypes,AA,AG and GG.The genotype frequencies of AA,AG and GG at FUT1 locus were 0.235,0.609 and 0.156,respectively.The allele A was dominant allele,its frequency was 0.540.This locus deviates from Hardy-Weinberg equilibrium（P0.01） in above foundation population.The individuals with resistant genotype AA were significantly higher than those individuals with AG and GG genotypes（P0.05）in HGB and WTBC,while there were no significant difference between AG and GG genotypes（P0.05）.The individuals with resistant genotype AA were significantly lower than those individuals with AG and GG genotypes（P0.05）in heterophil/lymphocyte（H/L） ratio,while there were no significant difference between AG and GG genotypes（P0.05）.For SRBC antibody titer,there were no significant difference among AA,AG and GG genotypes（P0.05）.These results demonstrated that the resistant populations to ETEC F18 with genotype AA also showed fine characters of stress resistance,AA genotype had good genetic effects on disease resistance traits of PWD and ED,as well as normal disease resistance.

关 键 词：FUT1基因 F18大肠杆菌 免疫指标 苏太猪 抗病育种 

分 类 号：S828[农业科学—畜牧学] Q343.15[农业科学—畜牧兽医]