规模化猪场戊型肝炎病毒感染状况的调查与分析  

Epidemiological Survey of Hepatitis E Virus in Industralized Swine Herds

作　　者：张厚勇[1] 陈东升[1] 张懿[1] 伍宜权 何启盖[1] 刘正飞[1] 

机构地区：[1]华中农业大学动物医学院农业微生物学国家重点实验室,武汉430070

出　　处：《畜牧兽医学报》2010年第10期1268-1273,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：农业微生物学国家重点实验室自主课题资助;国家生猪现代产业技术体系（nycytx-009）

摘　　要：为调查规模化猪场中戊型肝炎病毒（HEV）感染和流行特征，首先在4个猪场采集血样80份，用酶联免疫吸附试验（EusA）检测抗-HEVIgG抗体。在此基础上，作者于2007—2009年采集规模化猪场肛拭子样品415份，及规模化猪场送检的临床病猪肝脏样品135份，应用巢式PCR（RT—nPCR）方法进行HEVRNA检测，将PCR扩增产物测序，利用Neighbour—joining法进行进化分析。结果：4个猪场80份血清中HEV阳性血清56份，抗一HEV抗体阳性率高达60．0％以上。8个规模化猪场中有5个猪场的肛拭子检测到HEV，肛拭子阳性率8．43％（35／415）；从临床送检的发病猪采集的肝脏样品中HEV阳性率为15．55％（21／135）；HEVRNA最早检出日龄为7d，最晚检出日龄为84d。遗传进化分析表明：测序的38株阳性毒株之间同源性为92％～100％，均属于HEV基因4型。其中，从肛拭子中分离的毒株（FJ445406）与猪源毒株DQ279091株处在同一分支上；而肝脏中分离的毒株（GQ202266．1）和人源毒株处在一个亚支，属于4型中的同一亚型，表明该毒株与人源毒株亲缘关系较近。本研究表明华中地区猪群中普遍存在HEV感染，阳性率较高。本研究为规模化猪场HEV的防控提供了依据。The infection and epidemical characterization of hepatitis E virus （HEV） in industrialized swine herds were investigated. Eighty serum samples collected from 4 pig farms were detected for anti-gG antibody by ELISA. Then from 2007 to 2009, clinical liver specimen and anal swabs were collected in Hubei province, Henan province, and Anhui province for HEV RNA detection by nest RT-PCR （RT-nPCR）, PCR products were sequenced, and neighbour-joining method was utilized for phylogenetic analysis. 56 out of 80 serum samples were anti-HEV IgG positive. 5 out of 8 pig farms were HEV RNA positive and the positive rate was 62. 50%. HEV RNA positive rate was 8. 4%（35/415） in anal swabs, while HEV RNA positive rate was 15.55% （21/135） in liver. The minimal detection age of piglet is 7 days old and the maximum detection date is 84 days old. Two typical sequences were submitted to GenBank, the accession number is GQ202266.1 for a liver origin isolate and FJ445406 for an anal swab isolate. Phylogenetic analysis shows that the homology among 38 positive strains is 92%-100%, all the isolated strains belong to the genotype 4. The isolated HEV from the rectal swab strain （FJ445406） is at the same branch with swine-origin strain DQ279091, but the strain from the liver sample （GQ202266.1） exists on the same sub-brunch with a human strain. Taken together, our study shows that HEV circulates in pigs herds in central China, generally speaking, HEV genotypes in this area remained stable and belong to the same genotype. Our data knowledge of HEV infection in industrialized pig farm and control of HEV spread and animal, especially pigs.

关 键 词：规模化猪场 HEV 流行病学 

分 类 号：S852.659.6[农业科学—基础兽医学] R512.6[农业科学—兽医学]