猪IFN-γ和CpG基序双因子重组质粒增强亚洲Ⅰ型口蹄疫灭活病毒抗原免疫水平的研究  

Enhanced Immunity to Killed FMDV Antigen by Dual Factors Recombinant Plasmid of Porcine Interferon-gamma and CpG Motifs

作　　者：赵娜[1] 陈国华[1] 房永祥[1] 李玩生[1] 贾怀杰[1] 景志忠[1] 

机构地区：[1]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部兽医公共卫生重点实验室,甘肃省动物寄生虫病重点实验室,兰州730046

出　　处：《畜牧兽医学报》2010年第10期1281-1289,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技支撑计划(2006BAD31B03);国家高技术研究发展计划(2006AA10A203);甘肃省科技支撑计划(0804NKCA076)

摘　　要：分析CpG基序与猪IFN-γ基因组合的重组表达质粒对口蹄疫灭活抗原的免疫佐剂效应。以亚洲Ⅰ型口蹄疫灭活病毒为抗原,与含有猪IFN-γ基因和CpG基序的重组质粒配伍,采用肌肉注射法免疫小鼠,加强免疫后检测口蹄疫特异性抗体和中和性抗体水平、T淋巴细胞增殖反应、体内细胞毒性T细胞杀伤反应以及细胞因子IL-4、IL-6和IFN-γ的分泌水平。发现猪IFN-γ和CpG基序双因子重组质粒相对单一的因子能诱导更强的体液免疫和细胞免疫反应水平,尤其在中和性抗体和抗原特异性CTL反应方面更为明显。猪IFN-γ和CpG基序在诱导小鼠抵抗亚洲Ⅰ型口蹄疫灭活病毒免疫方面具有良好的佐剂效应,其重组质粒可望成为一种有前途的新型免疫佐剂。The present experiment was performed to determine whether a recombinant expression plasmid of IFN-γ and CpG ODN co-administrated with FMD antigen can enhance the immunological response.The mice were vaccinated intramuscularly with the conventional FMD antigen along with a recombinant plasmid inserted porcine IFN-γ gene and CpG ODN sequence.Specific antibody and neutralizing antibody of FMDV,lymphocyte proliferation,specific cytotoxic T lymphocyte（CTL） reaction and the secretory cytokines were detected.Adjuvant effect of the combination compared with CpG ODN alone on the antigen was able to elicit much stronger humoral immunity and cellular immunity in mice vaccinated,especially increase the neutralizing antibody level and CTL roles.Porcine IFN-γ and CpG ODN can enhance the immunological response against the FDMV antigen in mice,the recombinant plasmid of porcine IFN-γ and CpG ODN is potential to be an alternative adjuvant for the future FMD vaccine.

关 键 词：Γ干扰素 CPG基序 口蹄疫病毒灭活抗原 免疫佐剂 

分 类 号：S852.659.3[农业科学—基础兽医学]