家兔脑缺血再灌注损伤后边缘系统一氧化氮合酶活性的变化及血塞通对其变化的影响  

Diversity of Total Activity of Nitric Oxidesynthase and Effect of Xuesaitongon the Diversity in Limbic System with Cerebral Ischemia Reperfusion Injury of Rabbits

作　　者：赵军[1] 尹逊河[1] 梁京芸[1] 谢有莲[1] 郭丽红[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《畜牧兽医学报》2010年第10期1312-1316,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金资助项目(30671498);山东省自然科学基金资助项目(Y2003D04)

摘　　要：为了研究家兔脑缺血再灌注损伤后边缘系统一氧化氮合酶(NOS)活性的变化及血塞通对其活性变化的影响,探讨血塞通对全脑缺血家兔的脑保护作用。选取3月龄哈白兔63只,体质量(1500±150)g,随机分为脑缺血治疗组、脑缺血未治疗组和对照组3组,手术建立家兔全脑缺血模型,应用生化检测技术测定脑缺血再灌注后不同时间边缘系统NOS活性变化及血塞通对其活性的影响。结果表明,边缘系统内NOS活性在脑缺血再灌注2h后开始升高,6h达到高峰,随后逐渐下降,24～96h活性持续下降,120h后恢复至正常水平;脑缺血治疗组下降幅度大、速度快,在96h即恢复至正常水平。缺血治疗组和正常对照组NOS的活性极显著低于缺血未治疗组(P<0.01)。结果提示,血塞通可以通过减弱NOS活性进而维持NO的生理含量,对全脑缺血再灌注损伤家兔有明显的脑保护作用。To research the diversity of total activity of nitric oxide synthase（NOS） and effect of Xuesaitong on that diversity in limbic system with cerebral ischemia reperfusion injury of rabbits,and to study the protection of Xuesaitong on cerebral ischemia reperfusion injury of rabbits.Sixty three 3-month-old Harbin White rabbits（（1 500±150）g） were divided in 3 groups：the cerebral ischemia treatment group,the cerebral ischemia untreatment group and control group,randormly.The analog of cerebral ischemia were established by operation and the divisity of total activity of NOS and the effect of Xuesaitong on it were determined by the biochemistry technique.The results indicated that the total activity of NOS increased at 2 h after cerebral ischemia treatment reperfusion,increased to the highest at 6 h,and then decreased from 24 h to 96 h,and recovered to normal level at 120 h last.The cerebral ischemia treatment group showed bigger extent to descend,and recovered to normal level at 96 h.The cerebral ischemia treatment group and control group is obviously lower than that in cerebral ischemia untreatment group（P0.01）.The results suggested that Xuesaitong could protect the brain tissue with cerebral ischemia reperfusion injury of rabbits by decreasing the activity of NOS and maintaining the content of NO.

关 键 词：血塞通 缺血再灌注 边缘系统 一氧化氮合酶活性 

分 类 号：S859.792[农业科学—临床兽医学]