海兰褐商品蛋雏鸡毛源性上皮细胞癌病理学诊断  

Pathologic Diagnosis of Trichogenic Epithelioma on Hyline Commercial Layer Chickens

作　　者：孙洪磊[1] 秦梅[1] 肖一红[1] 王健[1] 杨凤[1] 倪伟[1] 刘思当[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《畜牧兽医学报》2010年第10期1328-1332,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家公益性行业(农业)科研专项(200803019)

摘　　要：针对目前山东省出现的某些商品蛋雏鸡皮肤增生、结痂为特征的致死性皮肤病,进行了流行病学调查、剖检、病理组织学及免疫组织化学检测。结果显示:该病仅在某场家、某批次的海兰褐商品蛋雏鸡中发现,多在5日龄左右发现病变,病程1月左右,病鸡最终衰竭死亡,发病率1%～10%不等;病初可见皮肤多发性黄色结节,随后结节融合,导致皮肤增厚、结痂,内脏器官无肉眼可见肿瘤病变;病理组织学观察,真皮层存在不同分化状态的毛囊样瘤细胞集团,瘤细胞有明显的异型性,病灶呈浸润性生长,侵犯神经及真皮下肌肉组织,伴有肌样纤维结缔组织大量增生;病变部皮肤表皮细胞显著增生,增生的基底细胞也有一定的异型性;免疫组化检测,肿瘤细胞呈广谱细胞角蛋白(CK)阳性;波形蛋白(Vim)、高分子量细胞角蛋白(CKHMW)、低分子量细胞角蛋白(CKLMW)、P53、Ki-67阴性;真皮层增生的肌样纤维结缔组织主要为平滑肌。该皮肤肿瘤初步诊断为毛源性上皮细胞恶性肿瘤。According to the characteristics of fatal disease of hyperplasia of skin and scabby among commercial layer chickens occurring in Shangdong province recently,epidemiological investigation,anatomic pathological change and observation,and histological and immunohistochemical examinations were carried out in suspected cases.The results showed that the disease was only discovered in the Hyline layer chickens of some batches of some chicken factory,about 1%-10% incidence,occurring among 5-day chickens,sustaining one month or so,finally leading to death.Yellow nodules could be seen initially throughout the skin,followed by the coalesced nodules,manking pachyderma and scab;histological examination indicated that the abnormal trichogenic tumor cells was invading the whole thickness of the dermis including nerve and striated muscle,and it was generally surrounded by fibrous tissue as well;Epidermal cells of skin lesions had a significant hyperplasia,and hyperplastic basal cell was atypia to some extent.Immunohistochemical examination revealed that in most of the tumor cells,CK was positive;Vimentin,CK LMW,CK HMW,P53,Ki-67 were negative;the hyperplastic connective tissue in the dermis were masculine for α-SMA.It could be concluded that the disease might be diagnosed as trichogenic epithelioma.

关 键 词：海兰褐商品蛋鸡 毛源性肿瘤 病理学 免疫组织化学 诊断 

分 类 号：S858.1[农业科学—临床兽医学]