3种内源氨基酸测定方法的准确性比较分析  

The Comparative Analysis of Accuracy of Three Determining Endogenous Amino Acids Methods

作　　者：张鹤亮[1,2] 李德发[2] 谯仕彦[2] 张兆琴[1] 李亚奎[1] 

机构地区：[1]河北北方学院动物科技学院,张家口075000 [2]中国农业大学动物科技学院,北京100193

出　　处：《畜牧兽医学报》2010年第10期1346-1353,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：本试验采用无氮日粮法(PF法)、回归法(REG法)、高精氨酸法(HA法)测定生长猪回肠内源氨基酸流量,并以3种方法测定的内源氨基酸值对氨基酸回肠表观消化率(AID)进行校正,通过校正后消化率值随日粮蛋白质水平变化的平稳性来判断内源氨基酸测定值的准确性。选用体质量28kg的阉公猪6头,回肠末端安装简单"T"形瘘管。饲喂6种试验日粮,日粮粗蛋白水平分别为0、5%、10%、15%、20%和25%。采用6×6拉丁方试验设计。每期持续时间8d。其中前5d为日粮适应期,第6天连续采集食糜24h,以测定AID和采用PF法测定的内源氨基酸值计算回肠标准消化率(STID)。第8天每头猪日喂1次高精氨酸饲粮,然后连续24h采集回肠食糜,以便用HA法测定内源氨基酸值并计算回肠真消化率(RID)。试验结果表明,采用REG法测定的内源氨基酸流量,除甘氨酸、脯氨酸外,都比PF法的测定的值低(P<0.1);PF法与HA法比较,在10%～25%日粮蛋白质浓度范围内,低估了内源氨基酸的损失(P<0.01)。除苯丙氨酸、缬氨酸外,必需氨基酸AID随蛋白质水平增加呈二次性提高(P<0.05)。必需氨基酸STID随饲粮蛋白质水平的增加呈线性和二次性降低(P<0.05)。而RID在20%的饲粮粗蛋白水平内呈恒定平稳状态,完全不依赖于饲粮蛋白质水平(P>0.05)。本试验可得出以下结论:REG法和PF法低估了饲粮内源氨基酸损失,HA法是估计内源氨基酸损失的较准确方法。The aim of this study was to compare the accuracy of endogenous ileal amino acid loss values determined by regression（REG） method,the protein-free（PF） method and the homoarginine（HA） method.The endogenous ileal amino acid flows of growing swine determined using above methods were used to correct the apparent ileal amino acid digestibility（AID）,and then judge the accuracy of endogenous ileal amino acid values by stability of collected digestibility values with the change of dietary protein concentration.Six barrows,with an initial body weight of 28 kg,were surgically fitted with simple T-cannulae at the distal ileum and fed six experimental diets containing crude protein 0,5%,10%,15%,20%and 25% according to a 6 × 6 Latin-square design.Each experimental period lasted 8 days.On day 6 of each period,ileal digesta wascontinuously collected for 24 h to determine AID and standardized true ileal amino acid digestibility（STID） using PF method.On day 8,the pigs were given a single meal of the HA diets and their ileal digesta was collected for 24 h in order to determine the HA flow and calculate real ileal digestibility（RID）.The results showed that the values of endogenous ileal amino acid flow except glycine and proline determined using REG method were lower than those using PF method（P0.1）.Comparing with the HA method,PF method underestimated the loss of endogenous ileal amino acids（P0.01） in the range of 10%-25% of dietary protein concentration.The AID for all essential amino acids except phenylalanine and valine showed a quadratic increase（P0.05） with an increase in dierary protein.And STID decreased（P0.05） linely or quadraticly with an increase of dietary protein level.However,RID was stable and not influenced（P0.05） by dietary protein level within 20% of CP.In conclusion,REG method and PF method underestimated the loss of endogenous ileal amino acids and HA method was more accurate than REG or PF method for determining endogenous ileal amino acid loss.

关 键 词：猪 内源氨基酸 无氮日粮法 回归法 高精氨酸法 回肠表观消化率 回肠标准消化率 回肠真消化率 

分 类 号：S828.5[农业科学—畜牧学] S815.2[农业科学—畜牧兽医]